青岛大学学报（自然科学版）2026，Vol.39Issue(3)：15-23,9.DOI:10.3969/j.issn.1006-1037.2026.03.04
大肠杆菌DH5α的基因组甲基化图谱绘制及DNA甲基化在生长周期中的动态变化
Mapping the Genomic Methylation Landscape of Escherichia coli DH5α and Investigating the Dynamic Changes of DNA Methylation during the Growth Cycle
摘要
Abstract
The role of 5-methylcytosine(5mC)in bacterial gene expression regulation and genome architecture has not been fully elucidated.To address this gap,the growth curve of DH5α was plotted by measuring OD600 under M9 minimal medium conditions.The ex-pression of genes associated with the regulation of E.coli growth and proliferation at dif-ferent growth stages was examined using RT-qPCR.Furthermore,whole-genome bisul-fite sequencing(WGBS)was employed to generate comprehensive 5mC modification maps of Escherichia coli DH5α during exponential and stationary growth phases under M9 mini-mal medium conditions.The results show that dnaA,ftsZ,and SrsR are highly ex-pressed in exponential phase samples,whereas RpoS is highly expressed in stationary phase samples.WGBS data demonstrate the absence of detectable methylation in CpG con-texts and CHH motifs throughout the DH5α genome.However,consistent low-methyla-tion levels(4.2%～4.45%)are observed at CHG sites.Additionally,the methylation level in the stationary phase genome is slightly higher than that in the exponential phase.Furthermore,elevated methylation signals in the dnaA and ftsZ gene regions during the stationary phase suggest that increased methylation levels may play a role in suppressing gene expression.Conversely,5mC levels in the RpoS gene region are higher in the expo-nential phase than that in the stationary phase,implying that 5mC modification may be in-volved in the repression of RpoS gene expression during the exponential phase.关键词
大肠杆菌生长周期/5-甲基胞嘧啶/全基因组亚硫酸氢盐测序/表观遗传/基因组甲基化图谱Key words
Escherichia coli growth cycle/5-methylcytosine(5mC)/whole-genome bisul-fite sequencing(WGBS)/epigenetics/genomic methylation profile分类
医药卫生引用本文复制引用
王晓晗,李新敏..大肠杆菌DH5α的基因组甲基化图谱绘制及DNA甲基化在生长周期中的动态变化[J].青岛大学学报（自然科学版）,2026,39(3):15-23,9.基金项目
国家重点研发计划(批准号:2023YFD2403000)资助. （批准号:2023YFD2403000）
