中国海洋大学学报（自然科学版）2026，Vol.56Issue(10)：49-62,14.DOI:10.16441/j.cnki.hdxb.20250294
营养盐限制诱导的龙须菜卷曲分枝发育调控机制研究
Mechanisms of Curled Branch Development in Gracilariopsis lemaneiformis Induced by Nutrient Limitation
摘要
Abstract
In response to the abnormal branching of Gracilariopsis lemaneiformis observed in recent years in cultivation areas,this study investigated whether nutrient limitation could induce curly branch formation and preliminarily explored the mechanisms underlying this phenomenon.Thalli were cultured under different nitrogen and phosphorus levels,and changes in branch morphology were recorded.The induced curled branches were then characterized through morphological observation,physiological and biochemical measurements,and gene expression analysis.The results demonstrated that specific low-ni-trogen and low-phosphorus conditions consistently induced curly branch formation.This curled pheno-type was accompanied by disorganized cellular arrangement,reduced photosynthetic efficiency,and in-creased activities of oxidoreductase-related enzymes.Transcriptome analysis revealed significant down-regulation of photosynthesis-related pathways.Concurrently,up-regulation occurred in energy metabo-lism and oxidoreductase-related pathways.Taken together,these findings establish that nutrient limita-tion may affect branch development by altering photosynthesis,energy metabolism,oxidative status,and ROS-related signaling.These findings establish a mechanistic framework for elucidating branch de-velopment under nutrient limitation.关键词
龙须菜/营养盐限制/卷曲分枝/分枝发育/转录组/氧化压力Key words
Gracilariopsis lemaneiformis/nutrient limitation/curly branch/branching development/transcriptome/oxidative stress分类
生物科学引用本文复制引用
马子璇,季晓晓,李萍萍,隋正红,胡依依..营养盐限制诱导的龙须菜卷曲分枝发育调控机制研究[J].中国海洋大学学报（自然科学版）,2026,56(10):49-62,14.基金项目
国家自然科学基金项目(32503178) （32503178）
山东省自然科学基金项目(ZR2022QC105) （ZR2022QC105）
中央高校基本科研业务费专项(202313042) （202313042）
国家现代农业产业技术体系建设专项(CARS-50)资助Supported by the National Natural Science Foundation of China(32503178) （CARS-50）
the Shandong Provincial Natural Science Foundation(ZR2022QC105) （ZR2022QC105）
the Fundamental Research Funds for the Central Universities(202313042) （202313042）
the Special Project for the Construction of the National Modern Agricultural Industry Technology System(CARS-50) （CARS-50）
