青岛大学学报（医学版）2026，Vol.62Issue(3)：377-384,8.DOI:10.11712/jms.2096-5532.2026.62.078
CD46干扰对口腔鳞癌细胞LC3B及ATG5的调控效应
Regulatory effect of CD46 interference on microtubule-associated protein light chain 3B and autophagy-related protein 5 in oral squa-mous cell carcinoma cells
摘要
Abstract
Objective To investigate the regulatory effect of abnormal expression of CD46 and triggering receptor expressed on myeloid cells-1(TREM1)on microtubule-associated protein light chain 3B(LC3B)and autophagy-related protein 5(ATG5)in human oral squamous cell carcinoma(OSCC)HN30 cells.Methods Immunohistochemistry(IHC)was used to measure the ex-pression levels of CD46,TREM1,LC3B,and ATG5 in 29 OSCC tissue samples and 10 adjacent tissue samples.OSCC HN30 cells and human normal oral keratinocytes(HOKs)were used for in vitro research,and IHC was used to measure the expression of CD46 and TREM1 on cell surface.The lentiviral vector-mediated LV-shCD46 interference technique was used to establish an HN30 cell model with stable knockdown of CD46,and the group transfected with empty virus(LV-shNC)was used as the negative con-trol.Flow cytometry was used to validate transfection efficiency;RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of genes associated with autophagy and apoptosis before and after CD46 interference.A tumor-bearing mouse model was established,and the changes of tumor were dynamically monitored.IHC was used to measure the expression le-vels of CD46,TREM1,LC3B,and ATG5 in tumor.Results IHC results showed that the expression levels of CD46 and TREM1 on the surface of cancer tissue were significantly higher than those in adjacent tissue(x2=20.82,22.37;P＜0.01),while the expression levels of LC3B and ATG5 in cancer tissue were significantly lower than those in adjacent tissue(x2=6.97,7.11;P＜0.05);compared with HOKs,HN30 cells had higher expression levels of CD46 and TREM1 on cell surface.Flow cytometry showed a transfection efficiency of＞85％.RT-qPCR and Western blot showed that the LV-shCD46 group had significantly higher expression levels of genes associated with autophagy and apoptosis than the LV-shNC group(t=3.07-18.48,P＜0.05).In vivo experiments showed that compared with the LV-shNC group,the LV-shCD46 group had a significantly smaller tumor size(F=24.53,P＜0.05),significantly lower expression levels of CD46 and TREM1(t=38.70,25.95;P＜0.01),and significantly higher expression levels of LC3B and ATG5(t=21.20,33.74;P＜0.01).Conclusion CD46 and TREM1 are highly expressed on the surface of HN30 cells,and autophagy and apoptosis genes are inhibited.CD46 interference can activate autophagy and induce apoptosis of tumor cells.关键词
口腔肿瘤/补体膜辅助调节蛋白/髓系细胞触发受体-1/RNA干扰/自噬相关蛋白质类Key words
mouth neoplasms/membrane cofactor protein/triggering receptor expressed on myeloid cells-1/RNA inter-ference/autophagy-related proteins分类
医药卫生引用本文复制引用
刘晓丹,高美华,丛蓓蓓,王元非,吕晓芳,王万春..CD46干扰对口腔鳞癌细胞LC3B及ATG5的调控效应[J].青岛大学学报（医学版）,2026,62(3):377-384,8.基金项目
国家自然科学基金资助项目(82273190) （82273190）
青岛市医疗卫生重点学科建设项目(2025-2027) （2025-2027）
青岛市口腔疾病临床医学研究中心(22-3-7-lczx-7-nsh)和山东省医药卫生口腔内科学重点学科(青岛大学附属青岛市口腔医院)(2025-20-27)资助项目 （22-3-7-lczx-7-nsh）
