青岛大学学报（医学版）2026，Vol.62Issue(3)：393-399,7.DOI:10.11712/jms.2096-5532.2026.62.115
miR-34a通过抑制EMT信号通路调控胃癌生物学行为
MicroRNA-34a regulates the biological behavior of gastric cancer by inhibiting the epithelial-mesenchymal transition signaling pathway
摘要
Abstract
Objective To investigate the effect of microRNA-34a(miR-34a)on the malignant phenotypes(proliferation,apoptosis,cell cycle,invasion,and migration)of human gastric cancer cells SGC7901,as well as its regulatory role in epithelial-mesenchymal transition(EMT)and the Wnt/β-catenin pathway.Methods SGC7901 cells were randomly divided into control group(blank control),NC group(transfected with siRNA-NC),and mimic group(transfected with miR-34a mimic).Quantitative real-time PCR and Western Blot were used to measure the mRNA and protein expression levels of E-cadherin,Snail,Vimentin,β-catenin,glycogen synthase kinase-3β(GSK-3β),and c-Myc;CCK-8 assay was used to measure cell proliferation;flow cytome-try was used to assess cell cycle and apoptosis;Transwell assay and wound-healing assay were used to observe cell migration and invasion.Results Compared with the control group and the NC group,the mimic group had a significant increase in the expres-sion of miR-34a,a significant reduction in cell proliferation at 6-48 h of culture(F=577.82-4 724.87,P＜0.001),a significant increase in the proportion of cells in G0/G1 and S phases(F=8.41,156.70;P＜0.05),a significant reduction in the proportion of cells in G2/M phase(F=300.10,P＜0.001),a significant increase in cell apoptosis rate(F=48.92,P＜0.001),and significant re-ductions in cell migration and invasion capacities(F=106.80-198.40,P＜0.001),as well as significant increases in the mRNA and protein expression levels of E-cadherin and GSK-3β and significant reductions in the mRNA and protein expression levels of Snail,Vimentin,β-catenin,and c-Myc(F=15.12-781.20,P＜0.01),while there were no significant differences in these indica-tors between the control group and the NC group(P＞0.05).Conclusion This study shows that miR-34a exerts a tumor-sup-pressive effect on gastric cancer and can inhibit malignant tumor progression through a dual regulatory mechanism of suppressing EMT and blocking the Wnt pathway.关键词
胃肿瘤/微RNAs-34a/上皮-间质转化/Wnt信号通路Key words
stomach neoplasms/microRNAs-34a/epithelial-mesenchymal transition/Wnt signaling pathway分类
医药卫生引用本文复制引用
赵文文,井文君,冯青青,朱超,肖军,魏红梅..miR-34a通过抑制EMT信号通路调控胃癌生物学行为[J].青岛大学学报（医学版）,2026,62(3):393-399,7.基金项目
山东省中医药科技项目(Z20240222) （Z20240222）
青岛市中心医疗集团计划项目(PD-202102015) （PD-202102015）
