热带作物学报2026，Vol.47Issue(9)：2171-2179,9.DOI:10.3969/j.issn.1000-2561.2026.09.003
台湾金线莲内根-贝壳杉烯合酶基因克隆及红光诱导表达
Molecular Cloning and Expression of Ent Kaurene Synthase under Red Light Induction from Anoectochilus formosanus
摘要
Abstract
Ent kaurene synthase(KS)is a key rate-limiting enzyme in gibberellins(GAs)synthesis.The expression of the KS gene in Anoectochilus formosanus(A.formosanus)influences GAs synthesis and plant growth.In this paper,the AfKS gene open reading frame spans 2169 bp,encoding 723 amino acids.The AfKS gene expression was upregulated under red light stress,peaking at 42.38-fold higher than the control after 24 h.However,GAs were downregulated,with significant reductions in GA9,GA7,GA19 and GA8.Under red light conditions,the relative chlorophyll content in A.formosanus decreased(0.85 times),while MDA content increased(1.32 times).Nevertheless,red light stress elevated polysaccharide,total flavonoid,and free amino acid content by 1.13,1.02 and 1.34 times,respectively.After exogenous GAs added,relative chlorophyll content increased,MDA levels reduced,and polysaccharides,total flavonoids,and free amino acids elevated.This study would provide insights for further elucidating the mechanism of A.formosanus regu-lating GAs synthesis through KS under red light stress,thereby influencing plant stress resistance and secondary me-tabolite synthesis.关键词
内根-贝壳杉烯合酶(KS)/基因表达/红光诱导/台湾金线莲Key words
ent-kaurene synthase(KS)/gene expression/red light induction/Anoectochilus formosanus分类
农业科技引用本文复制引用
谢欣蕾,陈艳芳,訾菊如,张君诚,杨琳..台湾金线莲内根-贝壳杉烯合酶基因克隆及红光诱导表达[J].热带作物学报,2026,47(9):2171-2179,9.基金项目
福建省科技厅面上项目(No.2020J01382) （No.2020J01382）
福建省教育厅中青年教师教育科研项目(No.JAT220359). （No.JAT220359）
