药学研究2026，Vol.45Issue(8)：860-869,10.DOI:10.13506/j.cnki.jpr.2026.08.003
基于数据挖掘的清热解毒类方对炎症性肠病用药规律及作用机制
Research on the medication patterns and mechanisms of clearing heat and detoxifying prescriptions for inflammatory bowel disease based on data mining
摘要
Abstract
Objective Building on the demonstrated efficacy of traditional Chinese medicine(TCM)in inflammatory bowel disease(IBD)management,this study employed data mining to elucidate medication patterns of heat-clearing detoxifying formulas,predicting the mechanistic basis and validating the therapeutic efficacy of core herbal combinations.Methods Paper on effective heat-clearing and detoxifying herbal prescriptions for IBD was retrieved from the CNKI,VIP,and Wanfang databases.Frequency analysis,association analysis,and cluster analysis were performed on the herbs in the prescriptions to declare the medication patterns.Core herbs were selected based on association rules to construct a core herb-active ingredient-target interaction network.The enrichment analyses were conducted.Subsequently,the IBD-like inflammatory model was established by stimulating HT-29 cells with lipopolysaccharide(LPS,1 μg·mL-1).Following 24 h treatment with Pulsatilla chinensis-Coptis chinensis-Fraxinus rhynchophylla(BHQ,500 μg·mL-1),relative mRNA expression of IL-1β and TNF-α was quantified using reverse transcription quantitative PCR(RT-qPCR).Results A total of 86 articles were included,comprising 100 prescriptions and 204 herbal medicines,with 37 herbs showing the highest frequency.The analysis of medication patterns shows that there are more heat-clearing and bitter-cold drugs among these high-frequency drugs,most of which belong to the liver meridian.Association analysis of high-frequency herb clusters identified 24 highly supportive herb pairs,with the most significant two-herb combination being"Coptis chinensis-Fraxinus rhynchophylla"and the three-herb combination being"Pulsatilla chinensis-Coptis chinensis-Fraxinus rhynchophylla".Subsequently,69 targets were screened for the prevention and treatment of IBD with"Coptis chinensis-Fraxinus rhynchophylla",and 72 targets for the prevention and treatment of IBD with"Fraxinus rhynchophylla-Pulsatilla chinensis"-Coptis chinensis.The mechanism was primarily mediated through the PI3K/Akt pathway.The results showed that BHQ could significantly inhibit the increase of IL-1β and TNF-α levels in HT-29 cells induced by LPS,which revealed that BHQ could effectively inhibit the inflammatory response in IBD and play a therapeutic role.Conclusion Research indicates that the core herbal formula for IBD treatment using heat-clearing and detoxifying formulas consists of Pulsatilla chinensis,Coptis chinensis,and Fraxinus rhynchophylla(BHQ).Preliminary studies demonstrate that BHQ exhibits therapeutic effects on IBD,likely through its intervention in PI3K/Akt signaling pathways and subsequent regulation of inflammatory cytokines IL-1β and TNF-α.This study provides the first revelation of a"cooling and bitter liver-channel-dominant"medication pattern,offering theoretical support for clinical applications of clearing heat and detoxifying prescriptions in preventing and treating IBD.关键词
炎症性肠病/清热解毒/数据挖掘/白头翁/黄连/秦皮Key words
Inflammatory bowel disease/Clearing heat and detoxifying/Data mining/Pulsatilla chinensis/Coptis chinensis/Fraxinus rhynchophylla分类
医药卫生引用本文复制引用
马文珂,王嘉,张晓,时沙沙,姜昊,孙佳铮,曹萍,王克东,满帅..基于数据挖掘的清热解毒类方对炎症性肠病用药规律及作用机制[J].药学研究,2026,45(8):860-869,10.基金项目
山东省自然科学基金项目(No.ZR2022QH185) （No.ZR2022QH185）
