陕西中医2026，Vol.47Issue(9)：1162-1166,1194,6.DOI:10.3969/j.issn.1000-7369.2026.09.002
石芦清金颗粒调节IL-6/JAK2/STAT3通路对急性肺损伤小鼠炎症反应及氧化应激机制的影响
Effect of Shilu Qingjin granule on regulating the IL-6/JAK2/STAT3 pathway on inflammatory response in mice with acute lung injury
摘要
Abstract
Objective:To investigate the preventive and protective mechanisms of Shilu Qingjin granule on li-popolysaccharide induced acute lung injury(ALI)in mice by regulating the Janus kinase 2/transcriptional signaling transducer and activator 3(JAK2/STAT3)signaling pathway.Methods:Male C57BL/6 mice were randomly divided into 7 groups:blank group,model group,Lianhua Qingwen granule positive control group,methylprednisolone tablet positive control group,Shilu Qingjin granule low-dose group,Shilu Qingjin granule medium dose group,and Shilu Qingjin granule high-dose group,with 8 mice in each group.Each group was given the same volume of 0.9％sodium chloride solution,corresponding concentrations of Shilu Qingjin granule,Lianhua Qingwen granule,and methylpred-nisolone by gavage.After 7 days of administration,an ALI model was established by tracheal instillation of lipopo-lysaccharide.Observed the general condition of each group of mice;HE staining method was used to observe patho-logical changes in lung tissue;ELISA method was used to detect the levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in mouse serum;Western blot was used to detect the protein expression of JAK2,p-JAK2,STAT3,p-STAT3,and IL-6 in mouse lung tissue.Immunofluorescence assay was used to measure the levels of reac-tive oxygen species(ROS)in mouse lung tissue.Results:Compared with the blank group,typical ALI pathological changes such as infiltration of inflammatory cells and destruction of alveolar structural integrity were observed in the lung interstitium of the model group mice.The levels of TNF-α and IL-6 in the serum were significantly increased(P＜0.01),and the expression levels of p-JAK2 and p-STAT3 proteins were significantly increased(P＜0.01);Compared with the model group,the positive control group of methylprednisolone tablets,the positive control group of Lianhua Qingwen granule,and the low,medium,and high dose groups of Shilu Qingjin granule showed significant improvement in lung tissue structure damage,reduced inflammatory cell infiltration,significantly decreased TNF-αand IL-6 levels(P＜0.01),decreased p-JAK2/p-STAT3 expression(P＜0.05),and decreased ROS levels(P＜0.05).With the increase of Shilu Qingjin granule dosage used for intervention treatment in mice,the protein expression lev-els of p-JAK2 and p-STAT3 showed a significant downward trend(P＜0.01).Conclusion:Shilu Qingjin granule have a protective effect on ALI mice,and its mechanism may be related to the regulation of the JAK2/STAT3 signaling pathway.关键词
急性肺损伤/石芦清金颗粒/IL-6/JAK2/STAT3信号通路/小鼠/炎症/氧化应激Key words
Acute lung injury/Shilu Qingjin granule/IL-6/JAK2/STAT3 signaling pathway/Mice/Inflamma-tion/Oxidative stress分类
医药卫生引用本文复制引用
黄博杰,杨曼曼,孙一丹,王慧敏,崔嘉龙,程宏,屈小元..石芦清金颗粒调节IL-6/JAK2/STAT3通路对急性肺损伤小鼠炎症反应及氧化应激机制的影响[J].陕西中医,2026,47(9):1162-1166,1194,6.基金项目
陕西省科学技术厅重点研发计划项目(2020SF-344) （2020SF-344）
陕西省中医药管理局科研项目(SZY-KJCYC-2025-JC-044,LCPT019) （SZY-KJCYC-2025-JC-044,LCPT019）
