陕西中医2026，Vol.47Issue(9)：1167-1173,7.DOI:10.3969/j.issn.1000-7369.2026.09.003
糖肾灌肠方通过抑制TGF-β1/Smad通路改善糖尿病肾病肾脏纤维化的机制研究
Research on mechanism of Tangshen Enema formula in improving renal fibrosis in diabetic kidney disease by inhibiting TGF-β1/Smad pathway
摘要
Abstract
Objective:To explore the protective effect of the Tangshen enema formula on renal fibrosis in diabet-ic kidney disease(DKD)and its underlying mechanism.Methods:Forty adult C57BL/6J mice were divided into 4 groups:normal control group(10 mice),model group(10 mice),telmisartan group(10 mice),Tangshen Enema for-mula group(10 mice).Blood glucose,blood creatinine and urine microalbumin were measured,kidney index(KI)was calculated.Renal tissues were collected for light microscopy and electron microscopy observation.Western blot was used to detect the protein expression of transforming growth factor β1(TGF-β1),Smad3 and fibronectin(FN).RT-qPCR was used to detect the mRNA of TGF-β1,Smad3 and FN.The differences of the above indicators among the groups were compared.Results:The study found that compared with the normal control group,the levels of blood creatinine,urine microalbumin,KI,protein expression and mRNA of TGF-β1,Smad3 and FN in the model group were significantly increased(all P＜0.05),the renal tissue of the model group showed significant fibrotic pathological changes;Compared with the model group,the levels of blood creatinine,urine microalbumin,KI,the protein expres-sion and mRNA of TGF-β1,Smad3 and FN in the Tangshen Enema formula group were significantly decreased(all P＜0.05),the degree of renal fibrosis in the Tangshen Enema formula group was significantly alleviated.Conclusion:The study indicates that the Tangshen Enema formula improves the degree of renal fibrosis in DKD mice by regulating the TGF-β1/Smad3/FN signaling pathway.关键词
糖尿病肾病/糖肾灌肠方/转换生长因子β1/Smad/通路/肾脏纤维化Key words
Diabetic kidney disease/Tangshen Enema formula/Transformative growth factor β1/Smad/Path-way/Renal fibrosis分类
医药卫生引用本文复制引用
董丽明,王冲,马丽,李本皓,李惠,徐利娟,藏登,李寅辉..糖肾灌肠方通过抑制TGF-β1/Smad通路改善糖尿病肾病肾脏纤维化的机制研究[J].陕西中医,2026,47(9):1167-1173,7.基金项目
新疆维吾尔自治区自然科学基金资助面上项目(2025D01C204) （2025D01C204）
省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目(SKL-HIDCA-2021-35) （SKL-HIDCA-2021-35）
新疆医科大学附属中医医院院级课题重点项目(ZYY2020ZD05) （ZYY2020ZD05）
