陕西中医2026，Vol.47Issue(9)：1183-1188,6.DOI:10.3969/j.issn.1000-7369.2026.09.005
川芎嗪对妊娠期高血压模型大鼠子代NLRP3/Caspase-1通路及神经元凋亡的影响
Effects of ligustrazine on NLRP3/Caspase-1 pathway and neuronal apoptosis in offspring of pregnancy induced hypertension rats
摘要
Abstract
Objective:To investigate the effect of ligustrazine on neuronal apoptosis in offspring rats of pregnan-cy induced hypertension,and the role of nucleotide binding oligomerization domain-like receptor protein 3(NLRP3)/Cysteine aspartate protease-1(Caspase-1)pathway.Methods:SD pregnant rats were randomly divided into control group,model group,ligustrazine low-dose group(50 mg/kg),ligustrazine medium-dose group(100 mg/kg),ligus-trazine high-dose group(200 mg/kg)and magnesium sulfate control group(100 mg/kg).Except the control group,the other groups were injected with 7 mg/kg N-nitro-L-arginine methyl ester(L-NAME)from the 14th day of preg-nancy to construct the model of pregnancy induced hypertension in rats.From the 18th day,all groups were given corresponding drug treatment for 3 consecutive days.After the administration,measure the length and weight of the offspring rats.Water maze system was used to measure the cognitive function of offspring rats,hematoxylin eosin(HE)staining was used to observe the pathological changes of brain tissue in offspring rats,TUNEL method was used to detect the apoptotic index of neurons in the brain tissue of offspring rats and the protein expression levels of NLRP3,Caspase-1 and IL-1β were detected by Western blot.Results:Compared with those in the control group,the brain tissue structure of the offspring rats in the model group was seriously damaged,the cell arrangement was sparse,the cell bodies of neurons were reduced and deformed,and a large number of inflammatory cells were seen,the body length,body weight,neurological function score and times of crossing platform were significantly lower(P＜0.05),and the escape latency,neuronal apoptosis index and the expression of NLRP3,Caspase-1 and IL-1β protein were significantly higher(P＜0.05);compared with those in the model group,the damage degree of brain tissue structure,nerve cell damage and inflammatory cell infiltration of ligustrazine groups and magnesium sulfate treatment groups were significantly reduced,the body length,body weight,neurological function score and times of crossing platform were significantly higher(P＜0.05),and the escape latency,neuronal apoptosis index and the expression of NLRP3,Caspase-1 and IL-1β protein were significantly lower(P＜0.05).Moreover,there was no statistically signifi-cant difference in the detection indicators between the high-dose ligustrazine group and the magnesium sulfate control group(P＞0.05).Conclusion:Ligustrazine can reduce neuronal apoptosis and protect the neurological function of off-spring rats of gestational hypertension model.Its mechanism may be related to the inhibition of NLRP3/Caspase-1 signaling pathway.关键词
川芎嗪/妊娠期高血压/子代大鼠/NOD样受体蛋白3/半胱氨酸天冬氨酸蛋白酶-1/神经元凋亡Key words
Ligustrazine/Pregnancy induced hypertension/Offspring rats/NOD-like receptor protein 3/Cyste-ine aspartate protease-1/Neuronal apoptosis分类
医药卫生引用本文复制引用
何娟,吴洋,卓晓军..川芎嗪对妊娠期高血压模型大鼠子代NLRP3/Caspase-1通路及神经元凋亡的影响[J].陕西中医,2026,47(9):1183-1188,6.基金项目
湖南省卫生健康委员会科研计划项目(B202303017002) （B202303017002）
