世界针灸杂志（英文版）2026，Vol.36Issue(3)：289-298,10.DOI:10.1016/j.wjam.2026.05.001
隔药饼灸对腹泻型肠易激综合征大鼠结肠肥大细胞活化及结肠和脊神经节中TPSP,PAR2,PKC和TRPV1表达的影响
Effects of herb-cake-separated moxibustion on colonic mast cell activation and the expression of TPSP,PAR2,PKC,and TRPV1 in the colon and spinal ganglia of rats with diarrhea-predominant irritable bowel syndrome
摘要
Abstract
Objective:To investigate the potential mechanism by which herb-cake-separated moxibustion alleviates visceral hypersensitivity in rats with diarrhea-predominant irritable bowel syndrome(IBS-D),focusing on mast cell(MC)activation. Methods:Twenty SD rats were randomly divided into a blank group,a model group,a herb-cake-separated moxibustion group,and a modern medicine group,with 5 rats in each group.Except for the blank group,an IBS-D rat model was established using a combination of acetic acid enema and restraint tail-clipping stress.After successful modeling,the moxibustion group received herb-cake-separated mox-ibustion at"Ganshu(BL 19)","Pishu(BL 20)","Zusanli(ST 36)","Zhangmen(LR 13)",and"Qimen(LR 14)".The modern medicine group was administered ketotifen suspension(0.18 mg/kg)by gavage.Both interventions were performed once daily for 2 consecutive weeks.Body weight,Bristol stool form score,and abdominal withdrawal reflex(AWR)score were recorded before modeling,after modeling,and af-ter the intervention.After the intervention,toluidine blue staining was used to detect the number and morphology of MCs.Western blot was used to measure the protein expression levels of tryptase(TPSP),protease-activated receptor 2(PAR2),protein kinase C(PKC),and transient receptor potential vanilloid 1(TRPV1)in colon tissue and spinal ganglia.Immunofluorescence was used to detect the fluorescence expression of PAR2 and TRPV1 in colon tissue. Results:After the intervention,compared with the blank group,the model group showed significantly lower body weight and a significantly lower minimum volume threshold for achieving an AWR score of 3(P＜0.05),while the Bristol stool form score,MC count,and the protein expression levels of TPSP,PAR2,PKC,and TRPV1 in colon tissue and spinal ganglia were significantly increased(P＜0.05).Compared with the model group,the herb-cake-separated moxibustion group and the modern medicine group showed significantly increased body weight and a significantly increased minimum volume threshold for achiev-ing an AWR score of 3(P＜0.05),while the Bristol stool form score,MC count,and the protein expres-sion levels of TPSP,PAR2,PKC,and TRPV1 in colon tissue and spinal ganglia were significantly decreased(P＜0.05). Conclusion:Herb-cake-separated moxibustion can reduce visceral hypersensitivity and improve symp-toms such as abdominal pain and diarrhea in IBS-D model rats.Its mechanism may be associated with the regulation of MC activation and the downregulation of TPSP,PAR2,PKC,and TRPV1 protein expres-sion.关键词
Diarrhea-predominant irritable bowel syndrome/Herb-cake-separated moxibustion/Visceral hypersensitivity/Mast cells/Tryptase/Protease-activated receptor 2/Protein kinase C/Transient receptor potential vanilloid 1Key words
Diarrhea-predominant irritable bowel syndrome/Herb-cake-separated moxibustion/Visceral hypersensitivity/Mast cells/Tryptase/Protease-activated receptor 2/Protein kinase C/Transient receptor potential vanilloid 1引用本文复制引用
谢鑫,黄嘉怡,朱墨豪,刘密,张国山,刘未艾,常小荣,曹佳男..隔药饼灸对腹泻型肠易激综合征大鼠结肠肥大细胞活化及结肠和脊神经节中TPSP,PAR2,PKC和TRPV1表达的影响[J].世界针灸杂志（英文版）,2026,36(3):289-298,10.基金项目
Supported by National Natural Science Foundation of China:82374598 （）
Natural Science Foundation of Hunan Province:2024JJ6350 （）
Traditional Chinese Medicine Research Project of Hunan Province:B2024092 （）
University-Level Project of Hunan Uni-versity of Chinese Medicine:2022XYLH030 （）
Graduate Innovation Project of Hunan University of Chinese Medicine:2025CX169.The study sponsor had no role in the design and conduct of the study （）
collection,management,analysis,and interpretation of the data （）
preparation,review,or approval of the manuscript （）
and decision to submit the manuscript for publication. （）
