世界中医药2026，Vol.21Issue(11)：1961-1969,9.DOI:10.3969/j.issn.1673-7202.2026.11.001
龙脷叶75%乙醇提取物止咳作用及网络药理学研究
Antitussive Effects of 75％ Ethanol Extract of Sauropus spatulifolius Beille Leave and Network Pharmacology
摘要
Abstract
Objective:To investigate the antitussive effects and mechanisms of the 75%ethanol extract of Sauropus spatulifolius Beille leave.Methods:A cough model was established by capsaicin aerosol induction.The rats were randomly assigned into the blank group,model group,pentoxyverine citrate group,and high-,medium-,and low-dose groups of the 75%ethanol extract of S.spatulifolius leaves,with 6 rats in each group.The antitussive efficacy of the 75%ethanol extract of S.spatulifolius leaves was eval-uated by determining the cough inhibition rate in rats.Histopathological changes in rat lung tissues were observed using hematoxy-lin-eosin(HE)staining,and the levels of calcitonin gene-related peptide(CGRP)and neurokinin A(NKA)in lung tissues and ser-um were measured.Network pharmacology and molecular docking analyses were used to evaluate the mechanisms underlying the an-titussive effects of the 75%ethanol extract of S.spatulifolius leaves.Results:During the pharmacodynamic experiments,all rats in each group exhibited normal food and water intake,and no deaths occurred.Except for the model group,all other groups showed re-duced capsaicin-induced cough frequency,indicating good antitussive effects.Compared with the model group,the high-and medi-um-dose groups showed statistically significant differences(P<0.05),with increased organ indices,and lung consolidation was markedly improved in all administration groups.The concentrations of CGRP in lung tissues and serum of the pentoxyverine citrate group and medium-dose group were significantly lower than those in the model group,with statistically significant differences(P<0.05).Network pharmacology analysis identified 24 active compounds,399 key compound-related targets,key intersecting targets including signal transducer and activator of transcription 3(STAT3),proto-oncogene tyrosine-protein kinase Src(SRC),and heat shock protein 90 alpha family class A member 1(HSP90AA1),as well as 172 related Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathways.Molecular docking results showed that SRC had relatively high binding activity with apigenin,while epidermal growth factor receptor(EGFR)exhibited relatively high binding activity with 7-hydroxycoumarin,emodin,apigenin,and kaempferol.Conclusion:The 75%ethanol extract of S.spatulifolius leaves exhibits antitussive effects in capsaicin-induced cough rats and may exert its antitussive activity by reducing CGRP expression levels in lung tissues and serum.The underlying mecha-nisms may also involve the regulation of targets such as STAT3 and SRC by multiple components,including apigenin,EGFR-related compounds,7-hydroxycoumarin,emodin,and kaempferol.关键词
龙脷叶/70%乙醇提取物/止咳作用/作用机制/网络药理学/分子对接/CGRP/NKAKey words
Sauropus spatulifolius Beille leave/75%ethanol extract/Antitussive effect/Mechanism of action/Network pharma-cology/Molecular docking/CGRP/NKA分类
医药卫生引用本文复制引用
丘琴,韦红杏,覃芳芳,叶梅芳,甄丹丹,甄汉深,李钊文..龙脷叶75%乙醇提取物止咳作用及网络药理学研究[J].世界中医药,2026,21(11):1961-1969,9.基金项目
国家自然科学基金项目(81460601) （81460601）
壮瑶药协同创新中心(桂教科研[2013]20号) （桂教科研[2013]20号）
广西壮瑶药重点实验室(桂科基字[2014]32号) （桂科基字[2014]32号）
广西一流学科中药学(民族药学)(桂教科研[2018]12号) （民族药学）
广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号) （桂发改高技函[2020]2605号）
广西中医药大学"桂派杏林青年英才"培养项目(2022C032) （2022C032）
广西高等学校千名中青年骨干教师教育计划项目(桂教教师[2022]60号) （桂教教师[2022]60号）
广西科技基地和人才专项(桂科AD21238031) （桂科AD21238031）
广西重点研发计划项目(桂科AB21196016) （桂科AB21196016）
广西中医药重点学科壮药学(GZXK-Z-20-64) （GZXK-Z-20-64）
国家中医药管理局高水平中医药重点学科(壮药学)建设项目(国中医药人教函[2022]226号). （壮药学）
