世界中医药2026，Vol.21Issue(11)：1991-1998,2005,9.DOI:10.3969/j.issn.1673-7202.2026.11.004
健脾消癌方对结肠癌HCT116细胞转移的影响及机制研究
Effects and Mechanisms of Jianpi Xiaoai Prescription in Inhibiting Metastasis of Colorectal Cancer HCT116 Cells
摘要
Abstract
Objective:To investigate the effects of Jianpi Xiaoai Prescription on the metastasis of colorectal cancer HCT116 cells and its mechanisms.Methods:The effects of Jianpi Xiaoai Prescription on the concentration of exosomes secreted by HCT116 cells and the expression of miR-21 in exosomes were investigated.The cells were randomly divided into the high-,medium-,and low-dose Jianpi Xiaoai Prescription groups and the control group.After corresponding treatments,the proliferation ability of HCT116 cells was detected using the CCK-8 assay,the migration and invasion abilities were evaluated using the Transwell assay,and the protein ex-pression levels of extracellular signal-regulated kinase(ERK),phosphorylated extracellular signal-regulated kinase(p-ERK),signal transducer and activator of transcription 3(STAT3),phosphorylated signal transducer and activator of transcription 3(p-STAT3),and diacylglycerol kinase β(DGKB)were detected by Western blot.Results:Jianpi Xiaoai Prescription reduced the concentration of exosomes secreted by HCT116 cells and significantly inhibited the expression of miR-21 in exosomes(P<0.01).Compared with the control group,the proliferation ability of cells in the high-,medium-,and low-dose Jianpi Xiaoai Prescription groups was signifi-cantly decreased at 24,48,and 72 h(P<0.05).At the same time points,the cell invasion and migration rates in the high-,medi-um-,and low-dose groups were also lower than those in the control group(P<0.05),showing a dose-dependent trend.The expres-sion level of ERK in the medium-and high-dose groups was lower than that in the control group.The expression level of p-ERK in the low-,medium-,and high-dose groups was lower than that in the control group.The expression level of STAT3 in the medium-and high-dose groups was lower than that in the control group.The expression level of p-STAT3 in the low-,medium-,and high-dose groups was lower than that in the control group.The expression levels of DGKB in the low-,medium-,and high-dose groups were higher than those in the control group.All differences were statistically significant(P<0.05).Conclusion:Jianpi Xiaoai Prescrip-tion may inhibit the metastasis of colorectal cancer HCT116 cells by regulating the EV/miRNA/DGKB/ERK/STAT3 axis.关键词
健脾消癌方/结直肠癌/外泌体/增殖/侵袭/迁移/机制/EV/miRNA/DGKB/ERK/STAT3 轴Key words
Jianpi Xiaoai Prescription/Colorectal cancer/Exosomes/Proliferation/Invasion/Migration/Mechanism/EV/miR-NA/DGKB/ERK/STAT3 axis分类
医药卫生引用本文复制引用
何威华,吕俊,何兰..健脾消癌方对结肠癌HCT116细胞转移的影响及机制研究[J].世界中医药,2026,21(11):1991-1998,2005,9.基金项目
国家自然科学基金项目(82305329) （82305329）
湖北省中医药管理局中医药科研项目(ZY2023F054). （ZY2023F054）
