世界中医药2026，Vol.21Issue(11)：2006-2012,7.DOI:10.3969/j.issn.1673-7202.2026.11.006
参附注射液对慢性心力衰竭大鼠心肌细胞凋亡的作用机制研究
Mechanisms of Shenfu Injection on Cardiomyocyte Apoptosis in Rats with Chronic Heart Failure
摘要
Abstract
Objective:To investigate the effects of Shenfu Injection on cardiomyocyte apoptosis in chronic heart failure(CHF)and provide experimental evidence for drug-targeted therapy of CHF and the rational application of Shenfu Injection.Methods:Forty rats were randomly divided into the control group,model group,metoprolol group,and Shenfu Injection group according to a random number table method,with 10 rats in each group.A CHF rat model was established by intraperitoneal injection of adriamycin.After modeling,the general behavioral signs of rats were observed.Cardiac function was evaluated by echocardiography.Pathological changes in myocardial tissue were observed using hematoxylin-eosin(HE)staining.The protein expression levels of cysteine-aspar-tic acid protease-3(Caspase-3),cysteine-aspartic acid protease-9(Caspase-9),B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X protein(Bax)were detected by Western blot(WB).The contents of N-terminal pro-B-type natriuretic peptide(NT-proBNP),Caspase-3,Caspase-9,and Bax were determined by enzyme-linked immunosorbent assay(ELISA).The mRNA expression levels of Caspase-3,Bcl-2,and Bax were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR).Re-sults:Compared with the model group,the Shenfu Injection group showed improvements in general behavioral signs and pathological morphology,increased left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)(P<0.05),and decreased contents of NT-proBNP,Bax,Caspase-3,and Caspase-9(P<0.05).The mRNA expression levels of Caspase-3 and Bax were decreased(P<0.05).The protein expression levels of Caspase-3,Caspase-9,and Bax were decreased(P<0.05),whereas the protein expression level of Bcl-2 was increased(P<0.05).Conclusion:Shenfu Injection can effectively regulate apoptosis-re-lated factors,inhibit cardiomyocyte apoptosis,and delay the progression of CHF.关键词
慢性心力衰竭/细胞凋亡/阿霉素/参附注射液/线粒体凋亡通路/Caspase-3/Bcl-2/BaxKey words
Chronic heart failure/Apoptosis/Adriamycin/Shenfu Injection/Mitochondrial apoptotic pathway/Caspase-3/Bcl-2/Bax分类
医药卫生引用本文复制引用
叶嘉豪,刁承林,王荣,胡志希..参附注射液对慢性心力衰竭大鼠心肌细胞凋亡的作用机制研究[J].世界中医药,2026,21(11):2006-2012,7.基金项目
国家自然科学基金面上项目(82274412)——基于miR-155调控巨噬细胞极化探讨心肌缺血型心衰心肌纤维化病理机制及以方测证干预研究. （82274412）
