世界中医药2026，Vol.21Issue(12)：2157-2164,2172,9.DOI:10.3969/j.issn.1673-7202.2026.12.001
黑老虎根挥发油抗氧化活性部位筛选及化学成分解析
Screening of Antioxidant Active Fractions of Volatile Oil of Kadsurae Coccineae Radix and Chemical Composition Analysis
摘要
Abstract
Objective:To screen the antioxidant active fractions of the volatile oil from Kadsurae Coccineae Radix and analyze its chemical composition.Methods:The volatile oil of Kadsurae Coccineae Radix was extracted using seven methods.The antioxidant active fractions were evaluated by in vitro antioxidant assays,and their antioxidant chemical composition was analyzed using gas chromatography-mass spectrometry(GC-MS).Results:The antioxidant capacities of the volatile oil extracts of Kadsurae Coccineae Radix were ranked as follows:anhydrous ethanol extract＞methanol extract＞n-butanol extract＞ethyl acetate extract,while the an-tioxidant capacities of the n-hexane,petroleum ether,and dichloromethane extracts were relatively weak.A total of 41,38,42,49,44,41,and 40 compounds were identified in the volatile oil extracts of Kadsurae Coccineae Radix obtained using n-hexane,petrole-um ether,dichloromethane,ethyl acetate,n-butanol,anhydrous ethanol,and methanol,respectively.These compounds mainly includ-ed aromatic compounds,fatty compounds,and terpenoids.The common components with relatively high contents in the volatile oil extracts were dimethyl phthalate,methyl tetradecanoate,methyl hexadecanoate,methyl 2-hexylcyclopropaneoctanoate,methyl(Z,Z)-9,12-octadecadienoate,methyl trans-9-octadecenoate,methyl stearate,(Z,Z)-3,13-octadecadien-1-ol,and methyl 20-methylico-sanoate.Conclusion:The volatile oil extract of Kadsurae Coccineae Radix obtained with anhydrous ethanol exhibited the strongest antioxidant capacity.Methyl(Z,Z)-9,12-octadecadienoate,methyl trans-9-octadecenoate,and(Z,Z)-3,13-octadecadien-1-ol were the main components identified;however,the antioxidant capacities of the seven volatile oil extracts differed considerably.关键词
黑老虎根/挥发油/抗氧化/气相色谱串联质谱联用仪/化学成分/活性部位Key words
Kadsurae Coccineae Radix/Volatile oil/Antioxidant/GC-MS/Chemical composition/Active fraction分类
医药卫生引用本文复制引用
王飞娟,王燕,杨长花,刘峰,景鹏..黑老虎根挥发油抗氧化活性部位筛选及化学成分解析[J].世界中医药,2026,21(12):2157-2164,2172,9.基金项目
国家自然科学基金面上项目(82274095) （82274095）
陕西省科技厅创新能力支撑计划项目(2025JC-GXPT-024) （2025JC-GXPT-024）
陕西省教育厅青年团队建设科研计划项目(21JP012) （21JP012）
陕西省教育厅重点科学研究计划项目(22JY-006). （22JY-006）
