世界中医药2026，Vol.21Issue(12)：2165-2172,8.DOI:10.3969/j.issn.1673-7202.2026.12.002
结合灰色关联度优化鲜竹沥总酚酸的纯化工艺及其体外活性分析
Optimization of Purification Process for Total Phenolic Acids from Succus Bambusae by Grey Correlation Degree and Evaluation of Their in Vitro Activities
摘要
Abstract
Objective:To investigate the purification process of total phenolic acids from Succus Bambusae using macroporous ad-sorption resins and evaluate the in vitro antibacterial activity of the purified total phenolic acid fraction.Methods:Macroporous ad-sorption resins with different polarities were screened using grey relational analysis.A single-factor experiment was conducted to op-timize the loading volume,adsorption time,washing volume,ethanol elution volume fraction,and elution volume of Succus Bambus-ae.The total phenolic acid content of the purified and enriched freeze-dried product was determined,and its components were quali-tatively analyzed by GC-MS.The diameters of the inhibition zones produced by different concentrations of total phenolic acids a-gainst Staphylococcus aureus and Pseudomonas aeruginosa were determined.Results:Grey relational analysis showed that the AB-8 macroporous adsorption resin had the highest degree of correlation(0.91).The optimal purification process was as follows:a loading volume of 3.5 BV(43 mL),an adsorption time of 30 min,a washing volume of 5 BV,and elution with 35 mL of 70％ethanol.The antibacterial activity of the purified total phenolic acid fraction of Succus Bambusae against the two tested bacteria increased with increasing concentration.Conclusion:The optimized purification process is stable and feasible and can be used for the purification of total phenolic acids from Succus Bambusae.The purified product exhibits good antibacterial activity.关键词
鲜竹沥/灰色关联度法/总酚酸/分离纯化工艺/大孔吸附树脂/抑菌活性Key words
Succus Bambusae/Gray correlation method/Total phenolic acid/Purification process/Macroporous adsorption resin/Antibacterial activity分类
医药卫生引用本文复制引用
张娜,伍振峰,曹萱,张江山,张佳莹,孙冬妍,刘英,杨明,张雨恬..结合灰色关联度优化鲜竹沥总酚酸的纯化工艺及其体外活性分析[J].世界中医药,2026,21(12):2165-2172,8.基金项目
国家重点研发计划项目(2018YFC1707203) （2018YFC1707203）
江西省自然科学基金项目(20224BAB216103) （20224BAB216103）
全国重点实验室自主部署重点研发计划项目(20243BCC31008) （20243BCC31008）
中药科研实践创新训练项目(24KYCX-YB030,24KYCX-YB062) （24KYCX-YB030,24KYCX-YB062）
中药改良创新江西省重点实验室项目(2024SSY07131) （2024SSY07131）
景德镇市重大科技研发专项"揭榜挂帅"项目(景财科指[2022]41号) （景财科指[2022]41号）
江西中医药大学技术开发委托合同(53523807). （53523807）
