世界中医药2026，Vol.21Issue(12)：2173-2181,9.DOI:10.3969/j.issn.1673-7202.2026.12.003
十味咳喘方改善慢性阻塞性肺疾病气道黏液高分泌的作用机制
Mechanisms of Shiwei Kechuan Formula in Improving Airway Mucus Hypersecretion of Chronic Obstructive Pulmonary Disease
摘要
Abstract
Objective:To evaluate the expectorant,antitussive,and anti-inflammatory effects of Shiwei Kechuan Formula and ex-plore its mechanisms of action in improving airway mucus hypersecretion in chronic obstructive pulmonary disease(COPD).Meth-ods:Mice were administered Shiwei Kechuan Formula by gavage at crude drug doses of 8.5,17,and 34 g/kg.The expectorant,anti-tussive,and anti-inflammatory effects were evaluated using the phenol red airway excretion test,concentrated ammonium hydroxide-induced cough test,and xylene-induced ear swelling test,respectively.A COPD model was established by cigarette smoke exposure combined with airway instillation of lipopolysaccharide(LPS).The levels of mucins(MUC)5AC and MUC5B in bronchoalveolar lavage fluid(BALF)were measured by enzyme-linked immunosorbent assay(ELISA).The mRNA expression levels of MUC5AC and MUC5B in lung tissue were determined by quantitative reverse transcription polymerase chain reaction(RT-qPCR).The ex-pression levels of inhibitor of κB kinase β(IKKβ),nuclear factor-KB(NF-κB)p65,phosphorylated inhibitor of κBα(p-IκBα),and inhibitor of κBα(IκBα)in lung tissue were analyzed by Western blot(WB).Pathological changes in lung tissue were observed by hematoxylin-eosin(HE)staining.Results:Shiwei Kechuan Formula significantly increased phenol red airway excretion,prolonged the latency period of concentrated ammonium hydroxide-induced cough,reduced the number of coughs,and decreased the degree of xylene-induced ear swelling(P＜0.05).The formula significantly improved pathological changes in the lung tissue of COPD model mice,reduced MUC5AC and MUC5B levels in BALF,downregulated MUC5AC and MUC5B mRNA expression in lung tissue,inhib-ited IKKβ expression,reduced NF-κB p65 expression,decreased the phosphorylation level of Iκ Bα,anid inhibited its degradation(P＜0.05).Conclusion:Shiwei Kechuan Formula has significant expectorant,antitussive,and anti-inflammatory effects and can improve airway mucus hypersecretion in COPD model mice.This mechanism may be associated with inhibition of NF-κB signaling pathway activation.关键词
十味咳喘方/慢性阻塞性肺疾病/气道高分泌/黏蛋白/核因子κB/脂多糖/信号通路Key words
Shiwei Kechuan Formula/Chronic obstructive pulmonary disease/Airway mucus hypersecretion/Mucins/NF-κB/Lipopolysaccharide/Signaling pathway分类
医药卫生引用本文复制引用
余梦瑶,刘若阳,许晓燕,王腾飞,李芳,余德海,罗霞..十味咳喘方改善慢性阻塞性肺疾病气道黏液高分泌的作用机制[J].世界中医药,2026,21(12):2173-2181,9.基金项目
"重大新药创制"国家科技重大专项(2018ZX09731-009) （2018ZX09731-009）
四川省区域创新合作项目(2024YFHZ0084) （2024YFHZ0084）
四川省中医药管理局科学技术研究专项(2023ZD006) （2023ZD006）
四川省"天府万人计划"资助资金项目(川万人第407号) （川万人第407号）
成都市技术创新研发项目(2024-YF05-02627-SN). （2024-YF05-02627-SN）
