世界中医药2026，Vol.21Issue(12)：2198-2203,6.DOI:10.3969/j.issn.1673-7202.2026.12.006
钙调磷酸酶/转录因子EB抑制神经元凋亡对帕金森病的机制研究
Mechanisms of Inhibition of Neuronal Apoptosis via the Calcineurin/Transcription Factor EB Pathway in Parkinson's Disease
摘要
Abstract
Objective:To investigate the mechanisms by which the calcineurin/transcription factor EB(calcineurin/TFEB)path-way inhibits neuronal apoptosis through microRNA-325-3p to alleviate damage in a cell model of Parkinson's disease.Methods:A cell model was established using human neuroblastoma(SK-N-SH)cells induced with 100 μmol/L 1-methyl-4-phenylpyridinium ion(MPP+).The levels of TFEB,microRNA-325-3p,calcineurin,apoptosis rate,glutathione(GSH),malondialdehyde(MDA),cy-clophosphamide(CYC),dynactin 4(P62),glucose-regulated protein 78(GRP78),and light chain 3-Ⅱ/light chain 3-Ⅰ(LC3-Ⅱ/LC3-Ⅰ)were compared among the different groups.Results:Compared with the control group,the MPP+group showed higher lev-els of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ,and lower levels of TFEB,microRNA-325-3p,and GSH;the MPP++NC group showed higher apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ levels,and lower mi-croRNA-325-3p and GSH levels;the MPP++microRNA-325-3p group showed higher levels of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ,and lower levels of microRNA-325-3p and GSH;the MPP++si-NC group showed higher apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ levels,and lower TFEB and GSH levels;and the MPP++si-calci-neurin/TFEB group showed higher levels of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ,and lower levels of TFEB and GSH.Compared with the MPP+group,the MPP++microRNA-325-3p group showed higher levels of microR-NA-325-3p and GSH,and lower apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ levels;the MPP++si-calcineurin/TFEB group showed higher levels of TFEB and GSH,and lower levels of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ.Compared with the MPP++anti-NC group,the MPP++microRNA-325-3p+pcDNA group,MPP++anti-mi-croRNA-325-3p group,and MPP++microRNA-325-3p+pcDNA-calcineurin/TFEB group showed higher levels of TFEB,microR-NA-325-3p,and GSH,and lower levels of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ;compared with the MPP++anti-microRNA-325-3p group,the MPP++microRNA-325-3p+pcDNA-calcineurin/TFEB group showed higher levels of calcineurin,apoptosis rate,MDA,CYC,P62,GRP78,and LC3-Ⅱ/LC3-Ⅰ,and lower levels of TFEB,microRNA-325-3p,and GSH(P＜0.05).Conclusion:MicroRNA-325-3p interferes with the calcineurin/TFEB pathway to alleviate cellular oxidative stress,inhibit neuronal apoptosis,enhance cellular autophagy,and improve endoplasmic reticulum stress and damage in the cell model of Parkinson's disease.This may represent a potential molecular target for the treatment of Parkinson's disease and provide a relevant theoretical basis for the diagnosis and treatment of Parkinson's disease.关键词
钙调磷酸酶/转录因子EB/微小核糖核酸-325-3p/神经元凋亡/帕金森病细胞模型/谷胱甘肽/丙二醛Key words
Calcineurin/TFEB pathway/MicroRNA-325-3p/Neuron apoptosis/Parkinson's disease cell model/Glutathione/Malondialdehyde分类
医药卫生引用本文复制引用
班维固,卿鹏,滕秀英,陆丽娜,朱莹,齐辉..钙调磷酸酶/转录因子EB抑制神经元凋亡对帕金森病的机制研究[J].世界中医药,2026,21(12):2198-2203,6.基金项目
国家自然科学基金面上项目(21973926) （21973926）
黑龙江中医药管理局面上项目(ZHY2023-104). （ZHY2023-104）
