色谱2026，Vol.44Issue(9)：1074-1081,8.DOI:10.3724/SP.J.1123.2025.10020
仪器分析自主实验设计:在线固相萃取-高效液相色谱-柱后衍生测定蔬菜中痕量农药残留
Self-designed experiment in instrumental analysis:online solid phase extraction-high performance liquid chromatography-post column derivatization for the determination of trace pesticide residues in vegetables
摘要
Abstract
In line with modern educational concepts and the demand for innovative talents,a course focused on self-designed experiments has been incorporated into the traditional instrumental analysis curriculum.This initiative aims to enhance students'ability to apply knowledge effectively.While mastering instrument operation,students are encouraged to anticipate potential challenges during experiments.Under instructor guidance,students constructed an online system for solid-phase extraction coupled with liquid chromatography and post-column derivatization with fluorescence detection.They addressed real analytical needs such as improving sensitivity and precision,minimizing interference,and conducting rapid analyses of trace carbamate pesticides in vegetable samples.This self-designed liquid chromatography experiment course,tailored to students'learning characteristics and interests,successfully engages their initiative and fosters independent innovation capabilities.As a result,it also improves teaching effectiveness and enhances learning efficiency.关键词
自主设计实验/在线固相萃取/高效液相色谱/柱后衍生/农残分析Key words
self-designed experiment/online solid-phase extraction(online SPE)/high perfor-mance liquid chromatography(HPLC)/post-column derivatization/pesticide residue analysis分类
化学化工引用本文复制引用
李琰,刘韫滔,黄熙恩,周子易,王梓丘,王京,丁飞,南晶,唐安娜..仪器分析自主实验设计:在线固相萃取-高效液相色谱-柱后衍生测定蔬菜中痕量农药残留[J].色谱,2026,44(9):1074-1081,8.基金项目
南开大学实验教学改革项目(24NKZZYQ03). Nankai University Experimental Teaching Reform Project(No.24NKZZYQ03). （24NKZZYQ03）
