生物加工过程2026，Vol.24Issue(4)：362-368,7.DOI:10.3969/j.issn.1672-3678.2026.04.002
不同酶解条件对蛋白质组学酶解效率及鉴定定量的影响
Effects of different digestion conditions on the digestion efficiency and protein identification and quantification of proteomics
摘要
Abstract
To explore the effects of different enzyme amounts and digestion times significantly affect the peptide digestion efficiency,protein identification and quantification,and to optimize the workflow of proteomic sample preparation,filter-aided sample preparation(FASP)and in-gel digestion were applied separately.Digestion performance was evaluated under a variety of protein-to-trypsin mass ratios(25∶1,50∶1,and 100∶1)and digestion times(4,8,and 16 h),followed by comparative analysis of protein and peptide identification and quantification data.Although digestion efficiencies differed between the two digestion protocols across tested enzyme amounts and digestion times,no substantial discrepancies were observed for the number of identified proteins or quantitative results.Our studies also demonstrated that the conventional 16 h overnight digestion could be shortened to 4 h without compromising the quality of protein identification and quantification.Our protocol significantly reduced the sample preparation time and offered a useful guidance for proteomics laboratories.关键词
蛋白质组学/酶量/酶解时间/酶解效率Key words
proteomics/enzyme amount/digestion time/digestion efficiency分类
生物科学引用本文复制引用
张琪,刘栋..不同酶解条件对蛋白质组学酶解效率及鉴定定量的影响[J].生物加工过程,2026,24(4):362-368,7.基金项目
国家自然科学基金(32371205) （32371205）
