生物加工过程2026，Vol.24Issue(4)：369-375,7.DOI:10.3969/j.issn.1672-3678.2026.04.003
栀子愈伤组织的诱导增殖及其代谢物分析
Induction and proliferation of Gardenia jasminoides callus and its metabolites
摘要
Abstract
Pericarp,seeds and seed masses from Gardenia jasminoides fruits were evaluated as explants for callus induction.After optimizing the callus proliferation conditions,we compared the corresponding effects of solid culture flasks,liquid shake flasks and plant bioreactors on the proliferation of G.jasminoides callus.Liquid chromatography-mass spectrometry(LC-MS)was used to detect and analyze metabolites in G.jasminoides callus.As a result,the seed mass isolated from G.jasminoides fruit exhibited the highest callus induction efficiency,reaching 20.95%.The optimal medium for callus proliferation was MS medium supplemented with 1.5 mg/L 6-benzylaminopurine(6-BA),1.5 mg/L naphthaleneacetic acid(NAA),and 30 g/L sucrose.G.jasminoides callus displayed robust proliferation in solid culture flasks,shake flasks and plant bioreactors.A total of 1 281 metabolites were annotated in G.jasminoides callus through the screening with the COCOUNT database,including terpenoids,alkaloids,organic acids,amino acids and peptides,shikimates,phenylpropanoids and other classes.Overall,an efficient proliferation culture system was constructed for G.jasminoides callus,and the subsequent metabolomic profiling revealed that the callus accumulates a diverse array of bioactive natural constituents.Our study provides essential technical support for the resource-oriented utilization of G.jasminoides cell cultures.关键词
栀子/愈伤组织/诱导/增殖/代谢物Key words
Gardenia jasminoides/callus/induction/proliferation/metabolites分类
农业科技引用本文复制引用
金磊磊,陈集双,孙宇昊,焦玉瑾,苑飞跃,吴志华,潘奕玮,张雨果,张勍,刘馨雅..栀子愈伤组织的诱导增殖及其代谢物分析[J].生物加工过程,2026,24(4):369-375,7.基金项目
国家自然科学基金面上项目(82373981) （82373981）
江苏省大学生创新训练计划(202410291209Y) （202410291209Y）
