生物加工过程2026，Vol.24Issue(4)：440-449,10.DOI:10.3969/j.issn.1672-3678.2026.04.010
响应面法优化间型脉胞菌菌丝体蛋白提取工艺研究
The extraction process of Neurospora intermedia OK protein by response surface methodology
摘要
Abstract
In order to improve the extraction efficiency of Neurospora intermedia OK protein(NiOK),the enzymatic hydrolysis and extraction parameters were optimized for a sequential process including enzymatic cell wall degradation,alkaline dissolution and acid precipitation.Through single-factor experiments and response surface methodology,the isoelectric point of the OK protein was determined to be 3.8.The optimal conditions for enzymatic hydrolysis were a material-to-liquid ratio of 20∶1(mL/g),an enzyme concentration of 3.5%,and a hydrolysis duration of 1.5 hours.For the subsequent extraction process,the optimal conditions included an alkaline dissolution at pH of 9.5,an extraction duration of 3 hours,and an acid precipitation duration of 1 hour.Under these optimized conditions,the protein extraction yield reached 40.02%,with a purity of 79.93%.This study establishes a theoretical foundation for the efficient extraction of Neurospora intermedia OK protein.关键词
间型脉胞菌蛋白/菌丝体蛋白/酶解条件/提取条件/响应面优化Key words
Neurospora intermedia OK protein/mycoprotein/enzymatic hydrolysis conditions/extraction conditions/response surface optimization分类
轻工纺织引用本文复制引用
何子茵,戴意强,王喆,谢乐乐,蔡一鸣,杨贝贝,陈志坤,王坤,夏秀东..响应面法优化间型脉胞菌菌丝体蛋白提取工艺研究[J].生物加工过程,2026,24(4):440-449,10.基金项目
国家重点研发计划(2024YFF1106800) （2024YFF1106800）
