生物技术通报2026，Vol.42Issue(9)：93-106,14.DOI:10.13560/j.cnki.biotech.bull.1985.2026-0023
水稻DnaJ型锌指蛋白OsDJA10正调控叶绿体发育
DnaJ-type Zinc Finger Protein OsDJA10 Positively Regulates Chloroplast Development in Rice
摘要
Abstract
[Objective]The function of DnaJ-type zinc finger protein molecular chaperones in rice remains incompletely understood.Using the OsDJA10 gene(LOC_Os08g36140)as a model,this study investigates the molecular mechanisms underlying the albino phenotype in its knockout(KO)lines and the yellowing phenotype in its RNA interference(RNAi)lines.This study also evaluates the high-light tolerance of its overexpression(OE)lines,providing a theoretical reference for breeding stress-resistant rice varieties.[Method]CRISPR/Cas9 gene editing,RNAi,and Agrobacterium-mediated transformation were employed to generate knockout,knockdown,and overexpression lines,respectively.The biological functions of the OsDJA10 gene were elucidated by measuring photosynthetic pigments,chlorophyll fluorescence,chloroplast ultrastructure,antioxidant enzyme activities,relative water content and relative electrical conductivity of leaves and roots,and growth traits,combined with subcellular localization,RT-qPCR,and transcriptomic analysis.[Result]OsDJA10 knockout seedlings exhibited a white-leaf lethal phenotype,with near-complete absence of plant pigments,mature chloroplasts,and thylakoid stacks.Expression of genes related to chloroplast structure and development was significantly downregulated,and differentially expressed genes were significantly enriched in photosynthetic pathways.RNAi-treated plants exhibited leaf chlorosis,significantly reduced photosynthetic pigment content compared to wild-type Zhonghua 11,and markedly downregulated Fv/Fm and NPQ.Chloroplast number decreased significantly,volume shrank markedly,thylakoid stacks were remodeled,and expression of chloroplast structure and development-related genes was downregulated.OsDJA10-overexpressing lines showed significantly reduced plant height,panicle length,and flag leaf length,with no positive effect on yield;however,they exhibited significantly higher tolerance to high-light stress compared to wild-type Zhonghua 11.[Conclusion]The OsDJA10 gene positively regulates chloroplast development in rice by influencing photosynthetic gene transcription and photosynthetic organ stability,while enhancing tolerance to high-light stress.关键词
水稻/DnaJ型蛋白/叶绿体/转录/高光胁迫/OsDJA10/分子伴侣/光合作用Key words
rice/DnaJ-type protein/chloroplast/transcription/high light stress/OsDJA10/molecular chaperone/photosynthesis引用本文复制引用
任琼,钟娇,段玉,秦童,康振辉..水稻DnaJ型锌指蛋白OsDJA10正调控叶绿体发育[J].生物技术通报,2026,42(9):93-106,14.基金项目
国家自然科学基金青年科学基金项目(31601291),五粮液集团有限公司与四川轻化工大学校地合作项目(CXY2021ZR010),四川轻化工大学人才引进项目(2024RC073),四川省科技厅项目(2020YFN0023) （31601291）
