山西医科大学学报2026，Vol.57Issue(8)：845-856,12.DOI:10.13753/j.issn.1007-6611.2026.08.001
基于网络药理学与实验验证探究阳和汤抑制乳腺癌的糖酵解机制
Mechanism of Yanghe decoction in inhibiting breast cancer via glycolysis based on network pharmacology and experimental validation
摘要
Abstract
Objective To investigate the potential mechanism of Yanghe decoction(YHT)in the treatment of triple-negative breast cancer.Methods ①Active components of YHT were screened using the TCMSP database,and disease targets were obtained from the GeneCards database.A component-target network was subsequently constructed,followed by molecular docking to validate the binding affinity between core components and key targets.②Sprague-Dawley(SD)rats were randomly divided into a control group and low-,medium-,and high-dose Yanghe Tang groups,which received intragastric administration of YHT at doses of 6,12,24 g/kg,respectively,twice daily(morning and evening)for 7 consecutive days.Blood samples were collected 1 h after the final administration,and sera were separated to obtain the corresponding medicated sera.MDA-MB-231 triple-negative breast cancer cells were then assigned to a control group(receiving 10%blank serum),and YHT low-,medium-,and high-dose groups(receiving 10%low-,medium-,and high-dose medicated serum,respectively).Cell proliferation viability was assessed by CCK-8 assay;cell migration and invasion were evaluated by Transwell assay;protein expression levels of E-Cadherin and N-Cadherin were detected by western blot;and glucose con-sumption and lactate production levels were also measured.③MDA-MB-231 cells were divided into a control group,a YHT high-dose group and a YHT high-dose+740Y-P(PI3K activator)group.Protein expression levels of p-PI3K,p-AKT,HIF-1α,HK2,and GLUT1 were examined by Western blot.Results ①Quercetin,neryl acetate,and oxyphenbutazone were identified as the core active compo-nents of YHT.Molecular docking demonstrated that these components exhibit favorable binding affinities for PI3K,AKT1,and HIF-1α proteins.②Compared with control group,the proliferation,migration,and invasion abilities of MDA-MB-231 triple-negative breast cancer cells significantly reduced in YHT low-,medium-,and high-dose groups(P<0.05);E-Cadherin expression was significantly up-regulated while N-Cadherin expression was significantly down-regulated(P<0.05);and glucose consumption and lactate production were markedly decreased(P<0.05).Moreover,compared with YHT low-and medium-dose groups,the above indicators in YHT high-dose group exhibited more pronounced changes(P<0.05).③Compared with control group,the protein expression levels of p-PI3K,p-AKT,HIF-1α,HK2,and GLUT1 in high-dose YHT group significantly decreased(all P<0.001).Compared with YHT high-dose group,protein levels of p-PI3K,p-AKT,HIF-1α,HK2,and GLUT1 significantly increased in YHT high-dose+740Y-P group(P<0.05).Conclusion YHT suppresses the migration,invasion,and epithelial-mesenchymal transition of triple-negative breast cancer cells and reduces the expression of glycolysis-related proteins,which may involve the inhibition of the PI3K/AKT/HIF-1α signaling axis.关键词
阳和汤/三阴性乳腺癌/网络药理学/分子对接/上皮-间质转化/细胞生物学功能/PI3K/AKT信号通路/糖酵解Key words
Yanghe decoction/triple-negative breast cancer/network pharmacology/molecular docking/epithelial-mesenchymal transition/cell biological function/PI3K/AKT signaling pathway/glycolysis分类
医药卫生引用本文复制引用
孙艾琳,李济宇,徐轶尔,孙贵才,范春香..基于网络药理学与实验验证探究阳和汤抑制乳腺癌的糖酵解机制[J].山西医科大学学报,2026,57(8):845-856,12.基金项目
上海市浦东新区名中医工作室建设项目(PDZY-2025-0720) （PDZY-2025-0720）
上海市浦东新区名中医继承人培养项目(PDZY-2025-0949) （PDZY-2025-0949）
复旦大学附属浦东医院引进人才项目(YJYJRC202305) （YJYJRC202305）
国家自然科学基金项目(82260870) （82260870）
