山西医科大学学报2026，Vol.57Issue(8)：867-874,8.DOI:10.13753/j.issn.1007-6611.2026.08.003
野百合碱诱导的右心衰竭大鼠模型中Piezo1表达上调与钙稳态调控蛋白表达异常共存
Co-existence of up-regulated Piezo1 expression and altered calcium homeostasis regulatory proteins in a rat model of monocrotaline-induced right ventricular failure
摘要
Abstract
Objective To observe the expression changes of the mechanosensitive ion channel Piezo1,the alterations in the phos-phorylation level of ryanodine receptor 2(RyR2),and the expression of sarco/endoplasmic reticulum calcium ATPase 2a(Serca2a)in right ventricular tissue of monocrotaline-induced right ventricular failure model.Methods Twelve Sprague-Dawley rats were randomly divided into control group(n=5)and model group(n=7).Rats in model group received a single intraperitoneal injection of monocrotaline(MCT,60 mg/kg)to establish a pulmonary arterial hypertension-induced right ventricular failure model,while those in control group received an equal volume of normal saline.Model establishment was validated by right ventricular catheterization,echocardiography,HE staining of lung tissues,Masson staining of right ventricle,and Western blot for fibrotic markers(Collagen Ⅰ and Collagen Ⅲ).Western blot was performed to detect the protein expression levels of Piezo1,Serca2a,total RyR2(T-RyR2),and phosphorylated RyR2(p-RyR2),and the p-RyR2/T-RyR2 ratio was calculated.Results Compared with control group,the right ventricular free wall thickness significantly increased(P<0.001),while the ratio of pulmonary acceleration time to ejection time decreased in model group(P<0.01);lung HE staining revealed disrupted alveolar structure and thickened pulmonary arteriolar walls in model group;the percentage of right ventricular fibrotic area was significantly elevated(P<0.01),and the protein expression levels of Collagen Ⅰ and Collagen Ⅲ were significantly up-regulated in model group(P<0.05);Piezo1 protein expression was significantly up-regulated in the right ventricular tissue in model group(P<0.000 1),Serca2a expression was significantly down-regulated(P<0.01),and the p-RyR2/T-RyR2 ratio significantly increased(P<0.01).Conclusion In the MCT-induced right heart failure model,the up-regulation of Piezo1 co-exists with down-regulated Serca2a and increased RyR2 phosphorylation in the right ventricular myocardium.关键词
肺动脉高压/右心衰竭/野百合碱/大鼠/Piezo1/钙稳态Key words
pulmonary arterial hypertension/right ventricular failure/monocrotaline/rats/Piezo1/calcium homeostasis分类
医药卫生引用本文复制引用
聂建,李倩,张静..野百合碱诱导的右心衰竭大鼠模型中Piezo1表达上调与钙稳态调控蛋白表达异常共存[J].山西医科大学学报,2026,57(8):867-874,8.基金项目
国家自然科学基金面上项目(2022JM-540) （2022JM-540）
