山西医科大学学报2026，Vol.57Issue(8)：915-921,7.DOI:10.13753/j.issn.1007-6611.2026.08.008
CD248在病理性瘢痕形成中的表达特征及作用
Expression profile and functional role of CD248 in pathological scarring
摘要
Abstract
Objective To investigate the expression characteristics and role of CD248 in pathological scar formation.Methods Sirius red staining,Masson staining,and immunohistochemical staining were employed to evaluate collagen deposition and CD248 level in normal skin tissues and scar tissues(mild and severe).RT-qPCR and Western blot were performed to detect CD248 mRNA and protein levels in normal skin-derived fibroblasts(NSF)and hypertrophic scar-derived fibroblasts(HSF),respectively.Western blot was used to measure CD248 protein levels in cell lysates and supernatants of HSF treated with matrix metalloproteinase inhibitors(Batimastat,S3304,and Cordycepin).ELISA was conducted to determine the levels of CD248,COL1A1,and COL3A1 in supernatants of NSF and HSF.Results Sirius red staining and Masson staining results revealed markedly increased collagen deposition in the dermis of hypertrophic scar tissues,with thick,dense collagen bundles and increased myofiber content,and these changes were more pro-nounced in severe scar tissues than in mild scar tissues.CD248 expression was up-regulated in scar tissues,and it was significantly higher in severe scars than in mild scars(P<0.05).Compared with NSF,CD248 expression was significantly elevated in HSF(P<0.01).CD248 was highly expressed in the supernatant of HSF;Batimastat significantly reduced supernatant CD248 level,while S3304 had no obvious effect,and Cordycepin markedly decreased but did not completely abolish CD248 level in the supernatant.The levels of CD248,COL1A1,and COL3A1 were significantly increased in HSF supernatants,with more pronounced elevations in HSF derived from severe scar tissues.Conclusion CD248 is highly expressed in scar tissues and HSF cells,and could be cleaved by matrix metalloproteinases 1,3,7 and then secrete into the supernatant.The secretion of COL1A1 and COL3A1 is increased in HSF cells.These findings suggest that CD248 may serve as a potential novel therapeutic target for patients with scars.关键词
瘢痕/CD248/成纤维细胞/细胞外基质/基质金属蛋白酶Key words
scar/CD248/fibroblasts/extracellular matrix/matrix metalloproteinase分类
医药卫生引用本文复制引用
毛康琳,魏艳..CD248在病理性瘢痕形成中的表达特征及作用[J].山西医科大学学报,2026,57(8):915-921,7.基金项目
陕西省自然科学基础研究计划项目(2024JC-YBQN-0783) （2024JC-YBQN-0783）
西安交通大学第一附属医院院基金项目(2025QN-15) （2025QN-15）
