现代检验医学杂志2026，Vol.41Issue(5)：20-25,6.DOI:10.3969/j.issn.1671-7414.2026.05.004
儿童手足口病患者EV-71和Cox-A16感染与血清miRNA谱特征的相关性研究
Study on the Correlation of EV-71 and Cox-A16 Infections with Serum miRNA Profiles in Pediatric Hand-Foot-and-Mouth Disease
摘要
Abstract
Objective To characterize the serum microRNA(miRNA)profile in pediatric hand-foot-and-mouth disease(HFMD)caused by Enterovirus 71(EV-71)or Coxsackievirus group A type 16(Cox-A16),and to analyze its clinical significance.Meth-ods Using a simple random sampling method,pediatric HFMD patients diagnosed at the Department of Pediatrics of Dazu Hos-pital Affiliated to Chongqing Medical University from November 2023 to August 2024 were enrolled.The discovery set included 8 children with EV-71 infection,and 8 with Cox-A16 infection.miRNA microarray was performed to screen for differentially expressed miRNAs.And the candidate miRNAs were analyzed by bioinformatics analysis.The validation set included Cox-A16 group,EV-71 group and healthy control group with 105 cases in each group.Serum miRNA levels were detected using real-time fluorescence quantitative polymerase chain reaction.Receiver operating characteristic(ROC)curves were used to develop a miR-NA-based diagnostic model.Results In the discovery set,five differentially expressed miRNAs(miR-21,miR-143,miR-494,miR-146a,and miR-221)were identified.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment anal-ysis confirmed that these differentially expressed miRNAs were involved in multiple immune-related pathways.In the validation set,serum levels of miR-21,miR-143,miR-494,miR-146a,and miR-221 were significantly higher in both EV-71 and Cox-A16 groups than in healthy controls(H=7.714～100.551,all P<0.05),and serum levels of miR-21,miR-494,miR-143,and miR-146a were significantly higher in the EV-71 group than the Cox-A16 group(Z=-37.215 to-9.443,all P<0.05).After adjusting for clinical variables including age,elevated levels of miR-21,miR-494,miR-143,miR-221,and miR-1466 remained significant-ly associated with the onset of HFMD(HR=1.104～1.400,all P<0.05).The five-miRNA panel showed diagnostic capability for HFMD with an area under ROC curve(AUC)values ranging from 0.584 to 0.874.Moreover,the combination of miR-21,miR-494,and miR-146a demonstrated the ability to differentiate between EV-71 and Cox-A16 single-virus subtype infections,with AUC 0.874.Conclusions Pediatric HFMD has a specific serum miRNA profile,and the three-miRNA panel(miR-21,miR-494,and miR-146a)shows potential for distinguishing EV-71 from Cox-A16 infections.关键词
手足口病/肠道病毒71型/柯萨奇病毒A组16型/儿童患者/微小核糖核酸Key words
hand-foot-mouth disease/enterovirus 71/Coxsackievirus group A type 16/pediatric patients/microRNA分类
医药卫生引用本文复制引用
周延,雷芳,杨阳,陈岑,王廷婷..儿童手足口病患者EV-71和Cox-A16感染与血清miRNA谱特征的相关性研究[J].现代检验医学杂志,2026,41(5):20-25,6.基金项目
重庆市科技发展项目(DZKJ2022JSYJ-KWXM1031). （DZKJ2022JSYJ-KWXM1031）
