现代检验医学杂志2026，Vol.41Issue(5)：46-51,6.DOI:10.3969/j.issn.1671-7414.2026.05.008
竹节参皂苷IVa调控DNMT1表达影响肝细胞癌细胞株增殖、迁移、侵袭及干性特征研究
The Research of Chikusetsusaponin IVa Regulated DNMT1 Expression to Affect Proliferation,Migration,Invasion,and Stemness of Hepatocellular Carcinoma Cells
摘要
Abstract
Objective To explore the function and mechanism of chikusetsusaponin IVa(CsIVa)in regulating the growth,metas-tasis,and stemness characteristics of hepatocellular carcinoma cells.Methods qRT-PCR and Western blot were used to detect the mRNA and protein expression of DNA Methyltransferase 1(DNMT1)in hepatocellular carcinoma cell lines HepG2,Huh7,Hep3B,and MHCC97-H,as well as in human hepatocytes LO2.HepG2 cells were divided into groups treated with 0,50,100,or 200 μmol/L CsIVa,and a group treated with 200 μmol/L CsIVa+DNMT1 overexpression.CCK-8 assay,flow cytometry,and cell spheroid formation assay were used to assess cell proliferation,apoptosis,and stemness characteristics,respectively.Transwell assay was used to evaluate cell migration and invasion abilities.Western blot was used to detect the protein expression of stemness markers(Nanog,Sox2,Oct4,KLF4,and CD133)in each group of cells.Results The expression levels of DNMT1 mRNA and protein in MHCC97-H,Hep3B,Huh7,and HepG2 were significantly higher than those in LO2(tmRNA=8.10～45.09,tprotein=4.63～22.66,all P<0.05).Compared with the CsIVa 0 μmol/L group,the CsIVa 50,100,and 200 μmol/L groups showed significantly reduced DNMT1 expression,cell proliferation,migration,invasion,and cell spheroid formation in HepG2 cells(t=4.32～36.18,all P<0.05),the protein levels of Nanog,Sox2,Oct4,KLF4,and CD133 were also significantly decreased(t=4.09～39.27,all P<0.05),and the apoptosis rate was significantly increased(t=7.12～18.34,all P<0.05).Compared with the CsIVa 200 μmol/L group,the CsIVa 200 μmol/L+DNMT1 group showed significantly increased DNMT1 expression,cell proliferation,migration,invasion,and cell spheroid formation in HepG2 cells(t=7.42～16.35,all P<0.05),the protein levels of Nanog,Sox2,Oct4,KLF4,and CD133 were also significantly increased(t=10.79～24.80,all P<0.05),and the apoptosis rate was significantly reduced(t=4.48,P<0.05).Conclusions CsIVa downregulates DNMT1 expression to inhibit the growth,and meta-static potential,and stemness characteristics of hepatocellular carcinoma cells.关键词
竹节参皂苷IVa/肝细胞癌/DNA甲基转移酶1/增殖/迁移/侵袭/干性特征Key words
Chikusetsusaponin IVa/hepatocellular carcinoma/DNA methyltransferase 1/proliferation/migration/invasion/stemness characteristics分类
医药卫生引用本文复制引用
冯变莹,陈静奇,郝湘文,宗鸣,陈皓,满秋红..竹节参皂苷IVa调控DNMT1表达影响肝细胞癌细胞株增殖、迁移、侵袭及干性特征研究[J].现代检验医学杂志,2026,41(5):46-51,6.基金项目
上海市第四人民医院科研启动专项(sykyqd02201). （sykyqd02201）
