现代检验医学杂志2026，Vol.41Issue(5)：52-57,70,7.DOI:10.3969/j.issn.1671-7414.2026.05.009
慢性鼻窦炎伴鼻息肉患者外周血与息肉组织CD8+T细胞免疫功能和组织蛋白学分析及与RASAL3关系研究
Analysis of CD8+T Cell Immune Function and Tissue Proteomics in Peripheral Blood and Polyp Tissue of Patients with Chronic Rhinosinusitis with Nasal Polyps and Their Relationship with RASAL3
摘要
Abstract
Objective This study investigated factors influencing CD8+T cell infiltration and immune function suppression in pe-ripheral blood and polyp tissues of patients with chronic rhinosinusitis with nasal polyps(CRSwNP).The correlation between these factors and Ras protein activator like 3(RASAL3)expression was examined to clarify the immunotherapeutic mechanisms of CRSwNP.Methods Twenty CRSwNP patients treated in the department of otolaryngology at Yunnan Provincial Hospital of Traditional Chinese Medicine from April 2023 to March 2024 were enrolled.Peripheral blood and nasal polyp tissues were col-lected.Twenty healthy individuals undergoing physical examinations served as the control group.Flow cytometry was used to analyze T lymphocyte subsets,CD8+T cell cytotoxic function,and expression of CD39 and PD-1 in T cells from both peripheral blood and polyp tissues.Tissue proteomic analysis was performed on polyp tissues from five randomly selected CRSwNP pa-tients and mucosal tissues from the uncinate process or turbinate of four patients with deviated nasal septum.Results Compared with the healthy control group,the CRSwNP peripheral blood group showed increased expression of granzyme B and perforin in CD8+T cells.Statistical significance was observed(t=3.229,2.133,both P<0.05).Compared with their own peripheral blood,the nasal polyp group exhibited a predominant increase in CD8+T cells(t=6.024,P<0.001).Expression of granzyme B and perforin decreased(u=11.000,38.000),while expression of CD39 and PD-1 increased(u=59.500,46.000),the differences were statistical-ly significant(all P<0.001).Tissue proteomic analysis identified 6 275 proteins.Compared with the deviated nasal septum group,470 proteins showed significant changes in the CRSwNP group,with 181 proteins upregulated.RASAL3 expression was significantly upregulated in the CRSwNP group(P<0.05).RASAL3 was found to negatively regulate the T cell receptor signaling pathway.Conclusions In CRSwNP nasal polyps,T lymphocyte subset distribution was characterized by a predominant increase in CD8+T cells.Elevated expression of CD39 and PD-1 correlated with reduced secretion of granzyme B and perforin by CD8+T cells,indicating an immunosuppressive state.Highly expressed RASAL3 in polyp tissues was involved in regulating CD8+T cell infiltration and functional suppression.关键词
慢性鼻窦炎伴鼻息肉/CD8+T细胞/组织蛋白学/Ras激活蛋白样3/流式细胞术Key words
chronic rhinosinusitis with nasal polyps/CD8+T cells/proteomics/Ras GTPase-activating protein-like 3/flow cytometry分类
医药卫生引用本文复制引用
刘骥,李亚波,黄春江,谭凌翔,刘益州..慢性鼻窦炎伴鼻息肉患者外周血与息肉组织CD8+T细胞免疫功能和组织蛋白学分析及与RASAL3关系研究[J].现代检验医学杂志,2026,41(5):52-57,70,7.基金项目
云南省科技厅中医联合专项面上项目(202301AZ070001-080) （202301AZ070001-080）
云南中医药大学校院联合基金项目(XYLH202201). （XYLH202201）
