陕西医学杂志2026，Vol.55Issue(9)：1202-1207,6.DOI:10.3969/j.issn.1000-7377.2026.09.007
丹参酮ⅡA调控微小RNA-155-5p/细胞因子信号抑制因子1/核因子-κB轴减轻小鼠动脉粥样硬化斑块炎症
Tanshinone ⅡA regulates the miR-155-5p/SOCS1/NF-κB axis to alleviate inflammation of atherosclerotic plaques in mice
摘要
Abstract
Objective:Exploring the effects and mechanism of tanshinone Ⅱ A on inflammation in mouse athero-sclerotic plaques.Methods:Fifty male apolipoprotein E-/-(ApoE-/-)mice were randomly divided into control group,model group,tanshinone ⅡA group,tanshinone ⅡA+agomir negative control(Agomir NC)group,and tan-shinone Ⅱ A+miR-155-5p Agomir group,with 10 mice in each group.Male ApoE-/-mice were fed on high-fat diet for 12 weeks to construct an atherosclerotic mouse model.Real-time fluorescent quantitative polymerase chain reac-tion(RT-qPCR)was used to detect the expression of miR-155-5p in aortic tissue.Enzyme-linked immunosorbent as-say(ELISA)kits were used to detect the levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),interleukin 6(IL-6),tumor necrosis factor α (TNF-α),IL-1β,and IL-10 in serum.Hematoxylin-eosin(HE)staining and oil red O staining were used to detect the pathological damage and lipid deposition of aortic tissue.Western blot was used to detect the expression of cluster of differentiation 86(CD86),inducible nitric oxide synthase(iNOS),CD206,arginase 1(Arg1)and suppressor of cyto-kine signaling 1(SOCS1)proteins and the phosphorylated nuclear factor-κB p65(p-NF-κB p65)/NF-κB p65 ratio in aortic tissue.Results:Compared with the control group,the miR-155-5p,TC,TG,LDL-C,aortic lipid deposition area,CD86,iNOS,IL-6,TNF-α,IL-1β,and p-NF-κB p65/NF-κB p65 ratio increased in the model group(P＜0.05),and HDL-C,CD206,Arg1,IL-10,SOCS1 decreased(all P＜0.05),and obvious atherosclerotic plaques were observed in the aorta.Compared with the model group,the above indicators in the Tanshinone ⅡA group showed significant im-provement(all P＜0.05).miR-155-5p Agomir partially reversed the improvement effect of Tanshinone ⅡA on plaque inflammation in atherosclerotic mice(P＜0.05).Conclusion:Tanshinone ⅡA might help reduce plaque in-flammation in atherosclerotic mice by downregulating M1-type and upregulating M2-type polarization-related protein expression,and its mechanism could be related to regulating the miR-155-5p/SOCS1/NF-κB axis.关键词
丹参酮ⅡA/动脉粥样硬化/微小核糖核酸-155-5p/细胞因子信号抑制因子1/核因子-κB/巨噬细胞极化Key words
Tanshinone ⅡA/Atherosclerosis/Mir-155-5p/SOCS1/NF-κB/Macrophage polarization分类
医药卫生引用本文复制引用
沈忱,马晓佳,关丽娜,何婷婷..丹参酮ⅡA调控微小RNA-155-5p/细胞因子信号抑制因子1/核因子-κB轴减轻小鼠动脉粥样硬化斑块炎症[J].陕西医学杂志,2026,55(9):1202-1207,6.基金项目
内蒙古自治区自然科学基金面上(联合)资助项目[2024MS(LH)0121] （联合）
