摘要
Abstract
Objective This experiment aimed to investigate mechanism by which Qiyin granules(QY),a herbal medicine,improve hepatic lipid metabolism in high-fat diet(HFD)-induced metabolic-associated fatty liver disease(MAFLD).Methods 60 C57BL/6J mice were randomly divided into control,model,pioglitazone/metformin-intervened,low,medium and high-dose Qiyin granules-intervened group,with 10 in each.Model was establish by HFD feeding,and interventions began at seven weeks for six weeks.Liver histopathological study was routinely conducted,and hepatic SIRT1/LXRα signaling pathway genes and their proteins were detected by Western blotting and qRT-PCR,respectively.Results Serum and hepatic triglycerides and total cholesterol levels in model group increased greatly as compared to in the control(P＜0.05),while they decreased obviously in pioglitazone/metformin-intervened,and low,medium and high-dose Qiyin granules-intervened group(P＜0.05);hepatic SIRT1 protein in pioglitazone/metformin-intervened,and low,medium and high-dose Qiyin granules-intervened group upregulated(P＜0.05),while hepatic LXRα,SREBP-1c and FASN protein expression down-regulated greatly as compared to in the model(P＜0.05);hepatic SIRT1 mRNA levels in pioglitazone/metformin-intervened,and low,medium and high-dose Qiyin granules-intervened group increased in a dose-dependent manner(P＜0.05),while hepatic LXRα,SREBP-1c and FASN mRNA levels decreased obviously(P＜0.05),as compared to in the model.Conclusions Qiyin granules could ameliorate HFD-induced MAFLD,maybe via modulating the SIRT1/LXRα signaling pathway,which might suppress de novo lipogenesis and attenuate inflammation.关键词
代谢相关脂肪性肝病/芪茵颗粒/SIRT1/LXRα/小鼠Key words
Metabolic-associated fatty liver disease/Qiyin granules,a herbal medicine/SIRT1/LXRα/Mice
