时珍国医国药2026，Vol.37Issue(17)：3201-3207,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1701
新加苁蓉菟丝汤通过FtMt/PINK1/Parkin调节人卵巢颗粒细胞线粒体铁超载抗铁死亡的分子机制
The molecular mechanism of Xinjia Congrong Tusi Decoction(新加苁蓉菟丝汤)in regulating mitochondrial iron overload to inhibit ferroptosis in human ovarian granulosa cells via the FtMt/PINK1/Parkin pathway
摘要
Abstract
Objective To explore the molecular mechanism of Xinjia Congrong Tusi Decoction(新加苁蓉菟丝汤,XCTD)in regulat-ing mitochondrial iron overload via the FtMt/PINK1/Parkin pathway,repairing the triptolide(TP)-induced dysfunction and damage of human ovarian granulosa cells(GCs),inhibiting ferroptosis,and promoting follicular development.Methods Cultured human ovarian granulosa cells(KGN cells)were divided into 7 groups:Group A:normal control(Control);Group B:TP model(TP);Group C:TP+10%drug serum XCTD(TP+XCTD);Group D:TP+Western medicine(TP+EV);Group E:XCTD+ferroptosis agonist(TP+XCTD+erastin);Group F:XCTD+ferroptosis agonist+NC(TP+XCTD+erastin+NC);Group G:XCTD+ferroptosis agonist+FtMt(TP+XCTD+erastin+FtMt).Lipid peroxidation in GCs was detected by flow cytometry;morphological changes of ferroptosis-related organelles in GCs were observed by transmission electron microscopy(TEM);changes in GCs and mitochondria were observed by nuclear fluorescence staining;total mitochondrial iron content and GSH expression in GCs were measured biochemically;and protein expression levels of FtMt,PINK1,Parkin,SLC7A11,and GPX4 in GCs were determined by Western blot(WB).Results Compared with Group B,lipid peroxidation levels in GCs were significantly lower in Groups C,D,and G.Compared with Group B,the morphol-ogy and structure of GCs recovered better under electron microscopy in Groups C,D,and G.Compared with Group B,nuclear fluores-cence intensity in GCs was significantly higher in Groups C,D,and G.Biochemical assays showed that mitochondrial iron content in GCs was significantly lower,while GSH levels were significantly higher in Groups C,D,and G compared to Group B.WB analysis revealed that,compared with Group B,the protein expression levels of FtMt,SLC7A11,and GPX4 in GCs were increased,while the levels of PINK1 and Parkin were decreased in Groups C,D,and G.Conclusion XCTD may inhibit ferroptosis in human ovarian granu-losa cells by regulating the FtMt/PINK1/Parkin pathway,thereby ameliorating mitochondrial iron overload.关键词
新加苁蓉菟丝汤/卵巢功能减退/FtMt/PINK1/Parkin通路/铁死亡/颗粒细胞Key words
Xinjia Congrong Tusi Decoction(新加苁蓉菟丝汤)/Ovarian hypofunction/FtMt/PINK1/Parkin Pathway/Ferroptosis/Granulosa cells分类
医药卫生引用本文复制引用
严谨,吴克明,张惜燕..新加苁蓉菟丝汤通过FtMt/PINK1/Parkin调节人卵巢颗粒细胞线粒体铁超载抗铁死亡的分子机制[J].时珍国医国药,2026,37(17):3201-3207,7.基金项目
国家自然科学基金(82405465) （82405465）
陕西省科技厅科研计划一般项目(2024JC-YBQN-0892) （2024JC-YBQN-0892）
陕西省教育厅重点科研计划项目(21JS017) （21JS017）
