时珍国医国药2026，Vol.37Issue(17)：3223-3227,5.DOI:10.70976/j.1008-0805.SZGYGY-2026-1704
炙红芪调节脾气虚大鼠肠道免疫的作用机制研究
Study on the regulatory mechanism of honey-processed Radix Hedysari on intestinal immu-nity in rats with spleen-qi deficiency
摘要
Abstract
Objective To investigate the mechanism of honey-processed Radix Hedysari(HRH)on intestinal immunity in rats with spleen qi deficiency(SQD).Methods The SQD rat model was established using a compound modeling method involving excessive fatigue,restricted diet,and purgation with bitter-coldherbs.HE staining was employed to observe the pathological damage of colonictis-sue in each group;ELISA was used to detect the expression levels of SIgA,CXCL1,and CXCL2 in colonic tissue homogenate;PCR was applied to determine the relative expression of IL-4 mRNA,IL-10 mRNA,IL-12 mRNA,PPARγ mRNA and TNF-α mRNA in colonic tissue of rats in each group.Results Compared with the blank group,the model group showed a significant decrease in the expression of colonic SIgA and the relative expression levels of IL-4 mRNA,IL-10mRNA,PPARγ mRNA,(P＜0.01),while theexpression levels of CXCL1,CXCL2,IL-12 mRNA,and TNF-α mRNA were significantly increased(P＜0.01).Compared with the model group,HRH could significantly increase the expression of colonic SIgA,IL-4 mRNA,IL-10 mRNA,and PPARγ mRNA in rats with SQD(P＜0.01),and inhibit the expression of CXCL1,CXCL2,IL-12 mRNA,and TNF-α mRNA(P＜0.05 or P＜0.01).Conclusion HRH can upregulate the level of SIgA in colonic tissue of rats with SQD,promote the relative expression levels of IL-4 mRNA,IL-10 mRNA,and PPARγ mRNA,thereby improving the intestinal immune function of rats with spleen qi deficiency.关键词
炙红芪/脾气虚/肠道免疫/趋化因子/PCR技术/作用机制Key words
Honey-processed Radix Hedysari/Spleen qi deficiency/Intestinal immunity/Chemokine/Polymerase chain reaction/Mechanism分类
医药卫生引用本文复制引用
辛二旦,李国峰,王彦钧,张育贵,边甜甜,孙宇靖,李越峰..炙红芪调节脾气虚大鼠肠道免疫的作用机制研究[J].时珍国医国药,2026,37(17):3223-3227,5.基金项目
国家自然科学基金(82160750) （82160750）
甘肃省教育厅产业支撑计划项目(2021CYZC-21) （2021CYZC-21）
甘肃省科技计划(创新基地与人才计划)基础研究创新群体项目(21JR7RA569) （创新基地与人才计划）
2024年省级人才项目(中药传统炮制技术传承创新团队建设与示范推广) （中药传统炮制技术传承创新团队建设与示范推广）
高校产业支撑计划项目(2024CYZC-41) （2024CYZC-41）
2024年度甘肃省联合科研基金项目(24JRRA882) （24JRRA882）
甘肃省研究生"创新之星"项目(2025CXZX-920) （2025CXZX-920）
