时珍国医国药2026，Vol.37Issue(17)：3244-3249,6.DOI:10.70976/j.1008-0805.SZGYGY-2026-1707
加味四君子汤调控PI3K/AKT/FoxO1信号通路对CTX诱导卵巢早衰大鼠的作用机制研究
Regulation of the PI3K/AKT/FoxO1 signaling pathway by Jiawei Sijunzi Decoction(加味四君子汤)in alleviating cyclophosphamide-induced premature ovarian failure in rats
摘要
Abstract
Objective To investigate the mechanism by which Jiawei Sijunzi Decoction(加味四君子汤,JSD)alleviates cyclophospha-mide(CTX)-induced premature ovarian failure(POF)in rats through regulation of the PI3K/AKT/FoxO1 signaling pathway.Methods Fifty rats were numbered and randomly divided into a blank group(n=10)and a model control group(n=40).The POF model was estab-lished by intraperitoneal injection of CTX.After successful modeling,the model rats were randomly divided into model group,low/medium/high-dose JSD groups(n=10 each).Serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),anti-Müllerian hormone(AMH),and gonadotropin-releasing hormone(GnRH)were measured by ELISA.Ovarian tissue mor-phology was observed by HE staining.Protein expression levels of PI3K,AKT,FoxO1,Bcl-2,Caspase-3,and Bax in ovarian tissues were detected by Western blot(WB).mRNA expression levels of PI3K,AKT,and FoxO1 in ovarian tissues were detected by q-PCR.Results Compared with the blank group,the number of atretic follicles,serum FSH and LH levels,as well as the protein and mRNA expression levels of PI3K,p-PI3K,AKT,p-AKT,Bax,and Caspase-3 in the model group were significantly increased(all P＜0.05),while serum E2 and AMH levels,and the protein and mRNA expression levels of Bcl-2 and FoxO1 were significantly decreased(all P＜0.05).Compared with the model group,the above indexes in the JSD dose groups showed the opposite trend(all P＜0.05).Conclusion JSD may alleviate CTX-induced premature ovarian failure by regulating the PI3K/AKT/FoxO1 signaling pathway,ameliorating ovarian tissue morphology and reducing excessive follicular atresia and depletion.关键词
PI3K/AKT/FoxO1信号通路/加味四君子汤/卵巢早衰/环磷酰胺Key words
PI3K/AKT/FoxO1 signaling pathway/Jiawei Sijunzi Decoction(加味四君子汤)/Premature ovarian failure/Cyclo-phosphamide分类
医药卫生引用本文复制引用
桑玉莹,辛如娟,代雅婷,李晶,王新斌..加味四君子汤调控PI3K/AKT/FoxO1信号通路对CTX诱导卵巢早衰大鼠的作用机制研究[J].时珍国医国药,2026,37(17):3244-3249,6.基金项目
甘肃省自然科学基金(23JRRA1206) （23JRRA1206）
