时珍国医国药2026，Vol.37Issue(17)：3258-3264,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1709
基于PINK1/Parkin线粒体自噬通路探讨疏肝温胆汤抗动脉粥样硬化的分子机制
Molecular mechanism of Shugan Wendan Decoction(疏肝温胆汤)against atherosclerosis via the PINK1/Parkin mitophagy pathway
摘要
Abstract
Objective To investigate the interventional effect and molecular mechanism of Shugan Wendan Decoction(疏肝温胆汤,SGWDD)on atherosclerosis(AS)through the PINK1/Parkin-mediated mitophagy pathway.Methods Forty ApoE-/-mice were randomly divided into four groups(n=10 per group):normal control,model,SGWDD,and atorvastatin(positive control)groups.After 12 weeks of treatment,body weight,serum lipid profiles(total cholesterol/TC,triglycerides/TG,low-density lipoprotein cholesterol/LDL-C,high-density lipoprotein cholesterol/HDL-C),inflammatory cytokines(tumor necrosis factor-α/TNF-α,interleukin-1β/IL-1β,IL-6,monocyte chemoattractant protein-1/MCP-1),and oxidative stress markers(superoxide dismutase/SOD,malondialdehyde/MDA,gluta-thione peroxidase/GSH-Px)were measured.Aortic morphology and mitochondrial ultrastructure were evaluated by hematoxylin-eosin(HE)staining and transmission electron microscopy(TEM),respectively.The expression of autophagy-related proteins(Beclin-1,PINK1,Parkin,P62,LC3II/LC3I)was detected by Western blot(WB)and reverse transcription quantitative polymerase chain reaction(RT-qPCR).Results Compared with the model group,both the SGWDD and atorvastatin groups showed significant improvements:attenuated dyslipidemia(decreased TC,TG,and LDL-C;increased HDL-C),reduced levels of inflammatory cytokines(TNF-α,IL-1β,IL-6,MCP-1),enhanced antioxidant capacity(increased SOD and GSH-Px;decreased MDA),and ameliorated aortic intimal thickening and lipid deposition.Furthermore,both treatments significantly promoted mitophagy,as evidenced by increased autophago-some formation,improved mitochondrial structure,upregulated protein and mRNA expression of Beclin-1,PINK1,Parkin,and LC3II/LC3I,and downregulated expression of P62(all P＜0.05).The effects of the two treatment groups were comparable.Conclusion SGWDD significantly ameliorates AS in ApoE-/-mice.The mechanism may be associated with the activation of the PINK1/Parkin-mediated mitophagy pathway,thereby reducing inflammatory response and oxidative stress.关键词
疏肝温胆汤/动脉粥样硬化/线粒体自噬/PINK1/Parkin通路Key words
Shugan Wendan Decoction(疏肝温胆汤)/Atherosclerosis/Mitophagy/PINK1/Parkin pathway分类
医药卫生引用本文复制引用
蔡海荣,蔡惠铃,胡瑶,黄金悦,林敏如,陈伯钧,夏晓莉..基于PINK1/Parkin线粒体自噬通路探讨疏肝温胆汤抗动脉粥样硬化的分子机制[J].时珍国医国药,2026,37(17):3258-3264,7.基金项目
国家自然科学基金面上项目(82074342) （82074342）
广州市科技计划项目(2024A04J3302) （2024A04J3302）
广东省中医药局科研项目(20233019,20251135,20264006) （20233019,20251135,20264006）
广东省中医院重点学科项目(01020245) （01020245）
