时珍国医国药2026，Vol.37Issue(18)：3409-3417,9.DOI:10.70976/j.1008-0805.SZGYGY-2026-1802
参芪泄浊饮通过Rab7/Caveolin-1/MMP-2信号通路对UUO大鼠肾脏纤维化的作用机制研究
Mechanism of Shenqi Xiezhuo Decoction(参芪泄浊饮)on renal fibrosis in UUO rats via Rab7/caveolin-1/MMP-2 signaling pathway
摘要
Abstract
Objective To observe the mechanistic role of Shenqi Xiezhuo Decoction(参芪泄浊饮,SQXZD)in renal interstitial fibrosis in unilateral ureteral obstruction(UUO)rats by affecting the Rab7/caveolin-1/MMP-2 signaling pathway.Methods After 7 days of adap-tive feeding,60 male Sprague-Dawley(SD)rats were randomly divided into the sham operation group,model group,a Niaoduqing gran-ule(NDQ)group,and low/medium/high-dose SQXZD groups,with 10 rats in each group.Except for those in the sham operation group,all rats underwent left ureteral ligation to establish the UUO rat model.After 14 days of successful model replication,intragastric administration was performed for a continuous 14-day intervention.Renal morphology was observed,and the levels of serum creatinine(Scr),blood urea nitrogen(BUN),serum uric acid(SUA),serum potassium(K⁺),serum sodium(Na⁺),and serum chloride(Cl⁻)were measured.Hematoxylin-eosin(HE)staining and Masson staining were employed to observe renal histopathological changes.Immunohistochemistry(IHC)was used to detect the expression of collagen-1,vimentin,and α-SMA proteins.The expression of caveo-lin-1,Rab7,and MMP-2 proteins was detected by Western blot(WB).Results Rats in the sham operation group exhibited normal physiological status.The model group showed signs of renal injury,including listlessness,emaciation,and hair loss.Renal function indicators and renal tissue fibrosis markers(vimentin,collagen-1,α-SMA)were abnormally elevated(P<0.01).Masson/HE staining revealed collagen deposition and destruction of glomerular/tubular structure.Following intervention with SQXZD,the physiological status and biochemical indicators of rats in each dose group and the NiDQ group were improved,with the most significant effects were observed in the high-dose group(P<0.01).The degree of renal fibrosis was alleviated,accompanied by decreased expression of caveo-lin-1 and Rab7 proteins and restoration of MMP-2 activity,confirming that this formula inhibits the progression of renal fibrosis by regu-lating the Rab7/caveolin-1/MMP-2 axis.Conclusion Shenqi Xiezhuo Decoction(参芪泄浊饮,SQXZD)can ameliorate renal function,renal interstitial fibrosis,and inflammatory responses in UUO rats.Its mechanism of action may be related to the downregulation of the renal interstitial fibrosis-associated Rab7/caveolin-1/MMP-2 signaling pathway.关键词
参芪泄浊饮/肾间质纤维化/Rab7/caveolin-1/MMP-2信号通路/自噬/炎症Key words
Shenqi Xiezhuo Decoction(参芪泄浊饮)/Renal interstitial fibrosis/Rab7/caveolin-1/MMP-2 signaling pathway/Autophagy/Inflammation分类
医药卫生引用本文复制引用
郭玲,朱昱霖,庞天霄,于宗民,何学红..参芪泄浊饮通过Rab7/Caveolin-1/MMP-2信号通路对UUO大鼠肾脏纤维化的作用机制研究[J].时珍国医国药,2026,37(18):3409-3417,9.基金项目
国家自然科学基金面上项目(81673956) （81673956）
国家自然科学基金青年基金(82004477) （82004477）
国家中医药管理局何学红全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
辽宁省自然科学基金计划面上项目(2024-MS-124) （2024-MS-124）
辽宁省教育厅科学研究项目(JYTMS20231819) （JYTMS20231819）
