时珍国医国药2026，Vol.37Issue(18)：3433-3440,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-1805
基于转录组学联合代谢组学探究异鼠李素防治肝纤维化的作用机制
Exploring the mechanism of isorhamnetin in preventing and treating hepatic fibrosis based on combined transcriptomic and metabolomic analysis
摘要
Abstract
Objective To elucidate the molecular mechanism of isorhamnetin in preventing and treating hepatic fibrosis through multi-omics integrated analysis,and to provide a scientific basis for its development and application.Methods KM mice were divided into a blank group,a hepatic fibrosis model group,and an isorhamnetin treatment group.Alterations in liver histology and function were assessed by hematoxylin-eosin(HE)staining,Masson's trichrome staining,Sirius red staining,and liver function tests.Transcrip-tomics and metabolomics were employed to detect differentially expressed genes and differential metabolites,respectively.Correlation analysis was performed between the differentially expressed genes(DEGs)and differentially abundant metabolites(DAMs).Molecular biology techniques were used to determine the expression of the differential molecules.Results HE,Masson's trichrome,and Sirius red staining,along with liver function tests,revealed that isorhamnetin significantly improved liver histopathological changes.Transcrip-tomic analysis identified 255 DEGs between the isorhamnetin and model groups.These DEGs were primarily enriched in signaling path-ways such as linoleic acid metabolism,arachidonic acid metabolism,glutathione metabolism,and unsaturated fatty acid biosynthesis.Metabolomic analysis revealed 451 DAMs between the isorhamnetin and model groups.These DAMs were enriched in multiple path-ways,mainly involving unsaturated fatty acid biosynthesis,ferroptosis,arachidonic acid metabolism,and autophagy.Following differ-ential analysis between the isorhamnetin and model groups,correlation analysis of the obtained DEGs and metabolites showed good model correlation,and they were primarily located in pathways such as unsaturated fatty acid biosynthesis.Molecular biology experi-ments confirmed that isorhamnetin promoted the expression of stearoyl-CoA desaturase 1(SCD1)and elongation of very long chain fatty acids protein 3(ELOVL3),and the difference was statistically significant compared to the model group.Conclusion Isorhamnetin may prevent and treat hepatic fibrosis by targeting SCD1 and ELOVL3 molecules and influencing unsaturated fatty acid metabolism.关键词
异鼠李素/肝纤维化/转录组学/代谢组学/不饱和脂肪酸Key words
Isorhamnetin/Hepatic fibrosis/Transcriptomics/Metabolomics/Unsaturated fatty acids分类
医药卫生引用本文复制引用
杨仕权,郑洋,肖华业,汪磊,赵依洋,赵铁建,王佳慧..基于转录组学联合代谢组学探究异鼠李素防治肝纤维化的作用机制[J].时珍国医国药,2026,37(18):3433-3440,8.基金项目
国家自然科学基金(82204755) （82204755）
广西自然科学基金(2023GXNSFBA026274,2024GXNSFAA010235) （2023GXNSFBA026274,2024GXNSFAA010235）
广西中医药大学赛恩斯新医药学院重点实验室(No.04:中医药壮瑶医药生物信息大数据研究) （No.04:中医药壮瑶医药生物信息大数据研究）
广西中医药大学赛恩斯新医药学院科研项目(2024ZZA002,2024ZZA003,2024ZZB006,2024ZZB007,2024ZZB010) （2024ZZA002,2024ZZA003,2024ZZB006,2024ZZB007,2024ZZB010）
广西中医药大学赛恩斯新医药学院大学生创新创业训练项目(202513643009) （202513643009）
