苏州科技大学学报（自然科学版）2026，Vol.43Issue(3)：61-69,9.DOI:10.12084/j.issn.2096-3289.2026.03.007
金属有机框架载体(Co-BTC)原位包封β-葡萄糖苷酶强化操作稳定性
In situ encapsulation of β-glucosidase in a metal-organic framework(Co-BTC)for enhanced operational stability
摘要
Abstract
β-glucosidase is a key enzyme in the cellulase system,but free enzymes are prone to inactivation under high temperature and organic solvent conditions.In this study,with metal-organic framework materials(Co-BTC)as the carrier,an immobilized enzyme composite material BGL@Co-BTC was prepared by in-situ co-precipitation method.The characterization results showed that the enzyme was successfully encapsulated and that the carrier struc-ture was stable.After immobilization by in-situ co-precipitation method,the thermal stability and solvent tolerance of β-glucosidase were significantly improved.It exhibited higher activity in 50%γ-valerolactone and 50%dimethyl sulfoxide.The results of cellulose dimer hydrolysis indicated that the conversion rate of BGL@Co-BTC at 60℃and 80℃was superior to that of the free enzyme,and that it had good recyclability and storage stability.This strategy provides a reference for the immobilization of key enzymes in the biotransformation of lignocellulose.关键词
β-葡萄糖苷酶/金属有机框架/纤维二糖Key words
β-glucosidase/metal-organic framework/cellobiose分类
能源科技引用本文复制引用
严立石,万健涛..金属有机框架载体(Co-BTC)原位包封β-葡萄糖苷酶强化操作稳定性[J].苏州科技大学学报（自然科学版）,2026,43(3):61-69,9.基金项目
苏州市产业创新项目(SNG202044) （SNG202044）
