天津中医药2026，Vol.43Issue(9)：1151-1160,10.DOI:10.11656/j.issn.1672-1519.2026.09.09
基于谱效关系分析白芍减轻雷公藤肝毒性的活性成分
Analysis of active components of Paeoniae Radix Alba to reduce hepatotoxicity of Tripterygium wilfordii based on spectrum-effect relationship
摘要
Abstract
[Objective]Based on the spectrum-effect relationship between the fingerprint of Paeoniae Radix Alba and its efficacy in attenuating the hepatotoxicity of Tripterygium wilfordii,this study aimed to screen the key active components in Paeoniae Radix Alba responsible for reducing Tripterygium wilfordii-induced liver damage,thereby providing a basis for elucidating the material foundation of its detoxification effect.[Methods]Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was employed to establish fingerprints for 20 batches of Paeoniae Radix Alba samples and identify common peaks.The hepatoprotective effect was evaluated using the survival rate of HepG2 cells as the pharmacodynamic index.Grey Relational Analysis(GRA),Principal Component Analysis(PCA),and Orthogonal Partial Least Squares-Discriminant Analysis(OPLS-DA)were comprehensively applied to screen potential active components.For the screened active components,multi-dimensional pharmacodynamic verification was further conducted through cell viability assays,morphological observation,and detection of alanine transaminase(ALT)and aspartate transaminase(AST)levels in the supernatant.[Results]The UPLC-Q-TOF-MS fingerprint was successfully established,with 17 common peaks calibrated and the corresponding components identified.Comprehensive analysis by GRA,PCA,and OPLS-DA revealed that gallic acid,oxypaeoniflorin,albiflorin,paeoniflorin,and benzoylpaeoniflorin were the key active components in Paeoniae Radix Alba for attenuating the hepatotoxicity of Tripterygium wilfordii.Cell experiments confirmed that all five compounds could significantly increase the survival rate of HepG2 cells induced by the main hepatotoxic component of Tripterygium wilfordii triptolide(TP),improve cell morphology,and reduce the levels of ALT and AST in the cell supernatant.[Conclusion]By integrating spectrum-effect relationship analysis with multi-dimensional pharmacodynamic verification,this study identified five active components from Paeoniae Radix Alba that attenuate the hepatotoxicity of Tripterygium wilfordii,providing a scientific basis for elucidating the material foundation of the detoxification effect of Paeoniae Radix Alba in combination therapy and its clinical application.关键词
白芍/雷公藤/谱效关系/肝毒性/活性成分/UPLC-Q-TOF-MSKey words
Paeoniae Radix Alba/Tripterygium wilfordii/spectrum-effect relationship/hepatotoxicity/active component/UPLC-Q-TOF-MS分类
医药卫生引用本文复制引用
马梦瑶,江瑾,李雯雯,李先宽,王金磊,王丽莉..基于谱效关系分析白芍减轻雷公藤肝毒性的活性成分[J].天津中医药,2026,43(9):1151-1160,10.基金项目
天津市卫生健康委员会中西医结合科研课题(2023076). （2023076）
