中国组织工程研究2026，Vol.30Issue(30)：7801-7808,8.DOI:10.12307/2026.957
聚己内酯/β-磷酸三钙支架联合骨髓间充质干细胞与骨形态发生蛋白2修复股骨缺损
Polycaprolactone/beta-tricalcium phosphate scaffold combined with bone marrow mesenchymal stem cells and bone morphogenetic protein-2 for femoral defect repair
摘要
Abstract
BACKGROUND:Polycaprolactone/β-tricalcium phosphate composite materials have shown good potential in bone tissue engineering,but the effect of their combined application with bone marrow mesenchymal stem cells and bone morphogenetic protein-2 for repairing large segmental bone defects needs systematic evaluation. OBJECTIVE:To investigate the in vitro osteogenic efficacy of 3D printed polycaprolactone/β-tricalcium phosphate scaffolds alone and in combination with bone marrow mesenchymal stem cells and bone morphogenetic protein-2,as well as their in vivo effect on the repair of large segmental femoral defects in rats. METHODS:Cylindrical polycaprolactone/β-tricalcium phosphate composite scaffolds(4 mm in diameter,6 mm in height)with a pore size of 500 μm and a porosity of 70％were prepared using fused deposition modeling 3D printing technology.Third-passage allogeneic rat bone marrow mesenchymal stem cells were seeded onto the scaffolds at a concentration of 1×106 cells/scaffold and cultured for 24 hours.Recombinant human bone morphogenetic protein-2(10 μg/scaffold)was loaded onto the scaffolds using a surface adsorption-freeze-drying method.Scaffold morphology,cytocompatibility,and osteogenic performance were detected in vitro.Rat models of mid-femoral large segmental bone defect(6 mm)were established and randomly divided into four groups(n=10 per group):polycaprolactone/β-tricalcium phosphate scaffold alone group(Group A),polycaprolactone/β-tricalcium phosphate scaffold+bone marrow mesenchymal stem cells group(Group B),polycaprolactone/β-tricalcium phosphate scaffold+bone morphogenetic protein-2 group(Group C),and polycaprolactone/β-tricalcium phosphate scaffold+bone marrow mesenchymal stem cells+bone morphogenetic protein-2 group(Group D).Postoperative general condition and survival rate of animals were observed.Specimens were collected at 4 and 8 weeks after surgery.Bone volume fraction and trabecular parameters were analyzed by Micro-CT scanning.Compressive strength and compressive modulusof the femur were detected bycompression test.New bone tissue morphology was observed by hematoxylin-eosin staining and Masson's trichrome staining.Runt-related transcription factor 2 expression was detected by immunofluorescence staining. RESULTS AND CONCLUSION:(1)One rat in Group A died due to deep anesthesia,and one rat in Group C was excluded due to incision infection.All rats in Groups B and D survived.(2)At 4 weeks after surgery,continuous callus bridging was observed in the bone defect area of Group D,and bone volume fraction and bone mineral density were higher than those in the other groups(P＜0.05).At 8 weeks after surgery,bone defects in Group D were completely healed,the bone volume fraction was significantly higher than that in Groups A,B,and C(all P＜0.05),the compressive strength recovered to approximately 75％of normal femur,and the compressive modulus recovered to approximately 68％.(3)Hematoxylin-eosin and Masson staining showed that the new bone trabeculae in Group D were thick and orderly arranged,with mature lamellar bone structure.The percentage of Runt-related transcription factor 2 immunofluorescence positive area and the expression levels of osteogenic genes(Runt-related transcription factor 2 and type Ⅰ collagen)were highest in Group D(P＜0.05).Overall,these findings indicate that polycaprolactone/β-tricalcium phosphate scaffold combined with bone marrow mesenchymal stem cells and bone morphogenetic protein-2 synergistically promotes early bridging and mechanical function reconstruction of large segmental femoral defects in rats,and this"scaffold-cell-growth factor"composite strategy provides an experimental basis for optimizing bone tissue engineering scaffolds.关键词
3D打印/聚己内酯/β-磷酸三钙支架/骨髓间充质干细胞/骨形态发生蛋白/大段骨缺损/组织工程Key words
3D printing/polycaprolactone/β-tricalcium phosphate scaffold/bone marrow mesenchymal stem cell/bone morphogenetic protein/large segmental bone defect/tissue engineering分类
医药卫生引用本文复制引用
覃国忠,李伟,牛军杰,李义凯,林文平,李丹丹,李军,柳鑫,曾凡宇..聚己内酯/β-磷酸三钙支架联合骨髓间充质干细胞与骨形态发生蛋白2修复股骨缺损[J].中国组织工程研究,2026,30(30):7801-7808,8.基金项目
深圳市坪山区卫生健康局课题(202201),课题名称:3D打印可降解微孔支架联合骨髓间充质干细胞和骨形态发生蛋白修复大段骨缺损的实验研究,项目负责人:覃国忠 （202201）
深圳市医疗卫生"三名工程"项目(SZZYSM202108013),项目负责人:李义凯 a grant from the Shenzhen Pingshan District Health Bureau,No.202201(to QGZ) （SZZYSM202108013）
Shenzhen Municipal"Sanming Enguneering"Project in Healthare,No.SZZYSM202108013(yo LYK) （yo LYK）
