现代中西医结合杂志2026，Vol.35Issue(15)：2045-2050,2056,7.DOI:10.3969/j.issn.1008-8849.2026.15.001
千金子制霜前后天然多元微乳对IEC-6细胞线粒体自噬性死亡的影响
Effects of natural microemulsion from Euphorbia Semen and Euphorbia Semen Pulveratum on mitochondrial autophagic death in IEC-6 cells
摘要
Abstract
Objective It is to explore the effects of natural microemulsion from Euphorbia Semen and Euphorbia Semen Pulveratum on mitochondrial autophagic death in rat intestinal epithelioid cell line No.6(IEC-6).Methods IEC-6 cells were divided into control group,Euphorbia Semen natural microemulsion group(50,100,200,400 and 800 μg/mL)and Euphorbia Semen Pulveratum natural microemulsion group(50,100,200,400 and 800 μg/mL),the formation of autoph-agic vesicles in IEC-6 cells was observed by MDC staining,the protein expressions of mitofusin 2(Mfn2),optic atrophy 1(OPA1),dynamin-related protein1(Drp1),microtubule-associated protein1 light chain 3-Ⅱ/Ⅰ(LC3-Ⅱ/Ⅰ)and se-questosome 1(p62)in the IEC-6 cells were determined by Western blot.In addition,IEC-6 cells were divided into the follow-ing groups:control group,3-MA group,Euphorbia Semen natural microemulsion group(50,100,200,400 and 800 μg/mL),3-MA+Euphorbia Semen natural microemulsion group(treated with respective dose of Euphorbia Semen natural microemul-sion after pretreatment with 10 μmol/L 3-MA),Euphorbia Semen Pulveratum natural microemulsion group(50,100,200,400,and 800 μg/mL),and 3-MA+Euphorbia Semen Pulveratum natural microemulsion group(treated with respective dose of Euphorbia Semen Pulveratum natural microemulsion after pretreatment with 10 μmol/L 3-MA),the IEC-6 cell via-bility in each group was detected by MTT.Results Compared with the control group,the number of autophagosomes and the relative intensity of green fluorescence in IEC-6 cells were significantly increased in all concentration groups of Euphorbia Se-men natural microemulsion(all P<0.05),the relative protein expressions of Mfn2,OPA1 and p62 in the cells were all sig-nificantly reduced(all P<0.05),while the relative protein expressions of Drp1,LC3-Ⅱ/Ⅰ(except for the 50 μg/mL concentration)were all significantly increased(all P<0.05).Compared with the corresponding concentration groups of Euphorbia Semen natural microemulsion,the number of autophagosomes in IEC-6 cells of the corresponding concentration groups of Euphorbia Semen Pulveratum natural microemulsion was significantly decreased,and the relative intensity of green fluorescence was significantly decreased(all P<0.05),the relative protein expressions of Mfn2,OPA1 and p62 in the cells were all significantly increased(all P<0.05),while the relative protein expressions of Drp1,LC3-Ⅱ/Ⅰ(except for the 50 μg/mL concentration)were all significantly decreased(all P<0.05).The IEC-6 cell survival rates in all corre-sponding concentration groups of 3-MA+Euphorbia Semen natural microemulsion were significantly higher than those in the corresponding concentration groups of Euphorbia Semen natural microemulsion(all P<0.05).Similarly,the IEC-6 cell survival rates in all corresponding concentration groups of 3-MA+Euphorbia Semen Pulveratum natural microemulsion were significantly higher than those in the corresponding concentration groups of Euphorbia Semen Pulveratum natural microemul-sion(all P<0.05).Conclusion Euphorbia Semen Pulveratum natural microemulsion induced cellular mitochondrial fu-sion/disintegration imbalance and autophagic cell death were significantly weaker than those induced by Euphorbia Semen natural microemulsion,which could effectively attenuate the cytotoxic effects of Euphorbia Semen natural microemulsion on IEC-6 cells.关键词
千金子/千金子霜/天然多元微乳/大鼠小肠隐窝上皮细胞/线粒体自噬性死亡Key words
Euphorbiae Semen/Euphorbia Semen Pulveratum/natural microemulsion/rat intestinal epithelioid cell/mitochondrial autophagic death分类
医药卫生引用本文复制引用
王慧楠,王英姿,朱海婷,张俊丽,王晓宇,陈文静,毛易凡,姜明瑞,张新宁,徐鹏..千金子制霜前后天然多元微乳对IEC-6细胞线粒体自噬性死亡的影响[J].现代中西医结合杂志,2026,35(15):2045-2050,2056,7.基金项目
北京市科技新星计划项目(20240484548) （20240484548）
中央高校基本科研业务费专项基金项目(2024-JYB-JBZD-047) （2024-JYB-JBZD-047）
国家自然科学基金资助项目(82374040) （82374040）
国家中医药管理局高水平建设学科项目(zyyzdxk-2023272) （zyyzdxk-2023272）
