畜牧与兽医2026，Vol.58Issue(10)：11-18,8.
乳酸杆菌发酵培养基的优化
Optimization of culture medium for Lactobacillus
摘要
Abstract
To overcome the bottleneck of large-scale cultivation in the industrial production of lactobacilli and to achieve cost-effective fer-mentation,this study systematically optimized the culture media for Lactobacillus plantarum LY19 and Lactobacillus johnsonii L63.Single-factor experiments were first conducted to identify appropriate types and concentrations of carbon sources,nitrogen sources,buffer salts,and trace elements.Subsequently,orthogonal experiments were employed to optimize the ratios of the key components.The results showed that the optimal culture medium formula for Lactobacillus plantarum LY19 was molasses 25 g/L,yeast extract 20 g/L,K2HPO4 2.5 g/L,and MgSO4 0.7 g/L.This formulation yielded a viable cell count of(9.48±0.07)lg CFU/mL at a material cost of approximately CNY 1.77 per liter.The optimal culture medium for Lactobacillus johnsonii L63 was bran 45 g/L,yeast extract 30 g/L,K2HPO4 2.5 g/L,and MgSO4 1.6 g/L,achieving a viable count of(9.26±0.16)lg CFU/mL with a material cost of approximately CNY 2.70 per liter.Both optimized media signif-icantly increased biomass production and at the same time reduce the cost by approximately 90％,compared with the laboratory-ready MRS medium(CNY 53.33 per liter).This study confirmed that tailored medium optimization based on specific strain characteristics would be an effective strategy to address large-scale cultivation challenges,providing an economically viable technical foundation for industrial application of lactobacilli in fields such as animal feed.关键词
植物乳杆菌/约氏乳杆菌/单因素试验/正交试验/培养基配方优化/成本Key words
Lactobacillus plantarum/Lactobacillus johnsonii/single-factor experiment/orthogonal experiment/medium optimization/cost分类
农业科技引用本文复制引用
倪纪萌,王兰,黄钰,许思雨,刘安琪,杭苏琴,丁立人..乳酸杆菌发酵培养基的优化[J].畜牧与兽医,2026,58(10):11-18,8.基金项目
海南省农垦投资控股集团有限公司2025年科技创新"揭榜挂帅"项目"利用椰子粕日粮生产高品质鸡蛋的关键技术及应用"[HKKJ2025JBGS14] （）
