石蕊 1卢猛 1吴锐 1田园 1吴小马 1黄克和2
作者信息
- 1. 江苏省协同医药生物工程有限责任公司,江苏 南京 211100
- 2. 江苏省协同医药生物工程有限责任公司,江苏 南京 211100||南京农业大学,江苏 南京 210014
- 折叠
摘要
Abstract
To compare the effects of different amounts of methionine on metabolic dysfunction-associated steatohepatitis(MASH)model induced by a choline-def high-fat purified diet and to provide support for clinical development of MASH drugs.Forty-eight healthy male SPF C57BL/6J mice were obtained and randomly divided into four groups based on their body weight,and the rodents were fed with methionine restricted choline deficient high fat diet(XTMRCD60),methionine choline deficient diet(XTMCD60),choline deficient high fat diet(XTCD60),and control diet(XTMCS10)for 6 weeks to establish MASH models.The results showed that,compared with the XTMCS10 group,the XTMRCD60 group had significantly elevated their liver index,serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),low density lipoprotein(LDL)levels(P＜0.05),significantly decreased their high density lipoprotein(HDL)level(P＜0.05)and significantly increased the levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in their liver tissue(P＜0.05).The mice in the XTCD60 group were obviously obese,with significantly increased serum ALT,AST,TG levels(P＜0.05),significantly decreased HDL level(P＜0.05),and significantly elevated liver inflammatory factor levels(P＜0.05).The XTMCD60 group significantly reduced their liver index(P＜0.05),significantly increased their serumALT,AST,VLDLlevels(P＜0.05),and significantly increased their inflammatory factor contents(P＜0.05).The HE staining and Oil Red O staining showed that,after 6 weeks of feeding with XTMRCD60,XTMCD60 and XTCD60,the mouse liver developed varying degrees of steatosis and inflammatory lesions,and the MASH models were successfully replicated.In conclusion,the three diet-induced mouse models had their own characteris-tics.Among them,the XTMRCD60 diet-induced MASH model possessed more advantages in modeling efficiency,integrity of pathological characteristics and animal welfare protection.关键词
代谢功能障碍相关脂肪性肝炎/蛋氨酸/胆碱/高脂/饲料Key words
metabolic dysfunction-associated steatohepatitis/methionine/cholinet/high fat/diet分类
农业科技
