畜牧与兽医2026，Vol.58Issue(10)：61-66,6.
北京地区牛乳房炎主要病原菌的分离鉴定及耐药性检测
Isolation and drug resistance analysis of main pathogens of bovine mastitis in the Beijing area
摘要
Abstract
This study was to investigate the distribution and antibacterial resistance of mastitis-related pathogens in large-scale dairy farms in the Beijing area.Bacterial count plates were used to screen the strains in milk samples,and the strains were identified by mass spectrome-try and 16S rDNA sequencing,and antimicrobial susceptibility test was performed on the pathogenic.The results were as follows:A total of 74 bacterial strains were isolated from the milk samples.The main pathogenic bacteria were Escherichia coli,Staphylococcus chromogenes,Staphylococcus sciuri,Streptococcus uberis,Streptococcus parauberis and Streptococcus dysgalactiae,with the isolation rates of 13.00%,11.00%,6.00%,6.00%,5.00%and 3.00%,respectively.The results of the antimicrobial susceptibility test showed that the resistance rates of Escherichia coli to amoxicillin-clavulanic acid,ceftiofur and kanamycin-cefalexin were 30.77%,30.77%and 7.69%,respectively.For Streptococcus uberis,the resistance rates to kanamycin-cefalexin and doxycycline were 16.67%and 33.33%,respectively.As for Strep-tococcus parauberis,the resistance rates to both kanamycin-cefalexin and marbofloxacin reached 20.00%.All the other strains were suscepti-ble to the tested antimicrobials.The results of the distribution and antimicrobial susceptibility test of pathogenic bacteria provided basic data support for prevention and treatment of bovine mastitis.关键词
奶牛乳房炎/病原菌/分离鉴定/药敏试验Key words
bovine mastitis/pathogens/isolation and identification/antimicrobial susceptibility test分类
农业科技引用本文复制引用
张小飞,刘思钰,杜继红,魏单平,孙丹,王亚芳,巩玉洁,李应超,高婷,冯研,巩浩..北京地区牛乳房炎主要病原菌的分离鉴定及耐药性检测[J].畜牧与兽医,2026,58(10):61-66,6.基金项目
北京市农业农村局综合保障项目(能力提升)(11000025Y000003335913) （能力提升）
