烟草科技2026，Vol.59Issue(9)：1-8,8.DOI:10.16135/j.issn1002-0861.2026.0087
烟秆碳点的制备及其抗烟草花叶病毒活性测定
Preparation of carbon dots from tobacco stalks and determination of their anti-tobacco mosaic virus activity
摘要
Abstract
To explore the preparation method of carbon dots from tobacco stalks and evaluate their anti-tobacco mosaic virus(TMV)activity,four types of biomass carbon dots were prepared via a one-step solvothermal method using tobacco stalks as carbon source and acetone,ultrapure water,ethanol and methanol as solvents,respectively.The microstructures,crystal structures,surface functional groups and electric charges of the carbon dots were characterized using transmission electron microscopy(TEM),X-ray diffraction(XRD),Fourier-transform infrared spectroscopy(FT-IR)and Zeta electric potential.Their optical properties were examined using ultraviolet-visible and fluorescence spectrophotometers,and their anti-TMV activity was evaluated using the detached leaf disc method.The results showed that:1)All four types of carbon dots exhibited a sphere-like structure with homogeneous dispersion and without significant agglomeration.Their surfaces were rich in hydrophilic functional groups such as carboxyl and hydroxyl groups and carried negative charges.Those structural features endowed carbon dots with good water solubility and stable optical properties,enabling them to emit sustained fluorescence under ultraviolet excitation.2)All carbon dot samples exhibited inhibitory effects against TMV,and their antiviral effects increased with increasing concentration.The carbon dot prepared with ethanol exhibited better antiviral activity.关键词
烟秆/生物质碳点/结构表征/抗烟草花叶病毒/活性Key words
Tobacco stalk/Biomass carbon dot/Structural characterization/Anti-tobacco mosaic virus/Activity分类
农业科技引用本文复制引用
王菊香,王俊美,昝盛翔,王宏伟,赵海云,周文兵,程桂广..烟秆碳点的制备及其抗烟草花叶病毒活性测定[J].烟草科技,2026,59(9):1-8,8.基金项目
云南省重大科技攻关项目"烟草内源抗病毒制剂抗病机理研究"(2024530000241009) （2024530000241009）
中国重大科技攻关项目/云南省重大科技攻关项目"基于抗病毒新产品开发的烟草废弃物资源化利用技术研究"[110202201021(LS‒05)/2023530000241013] （LS‒05）
昆明市春城人才计划"功效因子挖掘与利用"(2022SCP003). （2022SCP003）
