岩矿测试2026，Vol.45Issue(5)：1066-1076,11.DOI:10.15898/j.ykcs.202601080006
基于单色聚焦X射线荧光光谱与双层膜薄样技术的生物样品硒含量快速分析方法
Rapid Analysis Method of Selenium Content in Biological Sample Using Monochromatic Focused High-Precision X-ray Fluorescence Spectroscopy and a Double-Film Thin Sample Technique
摘要
Abstract
To address the challenges of complex sample pretreatment and the difficulty of on-site rapid screening for selenium(Se)detection in human hair,this study established a rapid analytical method based on monochromatic focused high-precision X-ray fluorescence spectroscopy(HPXRF).This work is motivated by the widespread Se deficiency in Chinese soils and its subsequent impact on human Se nutrition through the food chain.The proposed method employs high-sensitivity,low-background-noise HPXRF technology and simplifies sample pretreatment to merely cutting the hair into 1-2 mm segments.A custom-designed double-film thin sample preparation device was introduced to precisely control sample thickness,mass,and distribution,significantly improving measurement parallelism and accuracy.Systematic experiments demonstrated that the double-film thin sample method significantly enhanced sample uniformity and signal stability compared to conventional loose sample preparation,increasing the peak-to-background ratio of the Se characteristic peak by approximately 46%and effectively reducing matrix effects.A stable fluorescence signal was obtained with only 125 mg of sample,meeting the analysis requirements for trace biological samples.Samples prepared with this method exhibited good uniformity,enabling highly repeatable results without the need for a rotating platform.For core component selection,a 6 mm2 Si-PIN detector was chosen,as it satisfies the sensitivity requirements for trace Se detection while offering a significant cost-performance advantage.Method validation showed a good linear relationship for Se in the range of 314-2282 μg/kg,with a detection limit of 91 μg/kg.The rapid analysis method for Se in human hair established in this study is simple,rapid,and reliable,providing a feasible technical approach for the on-site rapid analysis of this element in such samples.The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202601080006.关键词
单色聚焦/高精度X射线荧光光谱(HPXRF)/双层膜薄样制样/非自旋模式/生物样品/硒Key words
monochromatic focusing/high precision X-ray fluorescence spectroscopy(HPXRF)/double-film thin sample preparation/non-spinning mode/biological sample/selenium分类
化学化工引用本文复制引用
张红平,杨晓曦,柏安露,蒋奡松,刘广,刘修明,李霞,宋硙..基于单色聚焦X射线荧光光谱与双层膜薄样技术的生物样品硒含量快速分析方法[J].岩矿测试,2026,45(5):1066-1076,11.基金项目
国家重点研发计划项目(2022YFC3700804) （2022YFC3700804）
