眼科新进展2026，Vol.46Issue(9)：694-699,6.DOI:10.13389/j.cnki.rao.2026.0120
桃叶珊瑚苷调节Ras/Raf/MEK/ERK通路对高糖诱导的视网膜神经节细胞炎性损伤的影响
Effect of aucubin on high glucose-induced inflammatory injury in retinal gan-glion cells by regulating the Ras/Raf/MEK/ERK pathway
摘要
Abstract
Objective To investigate the effect of aucubin(AU)on high glucose-induced inflammatory injury in reti-nal ganglion cells(RGCs)by modulating the Ras/Raf/MEK/ERK pathway.Methods RGCs were divided into the blank group(cultured with 5.5 mmol·L-1 glucose),the induction group(cultured with 30.0 mmol·L-1 glucose),the AU-L group(cultured with 30.0 mmol·L-1 glucose and 50 μmol·L-1 AU),the AU-H group(cultured with 30.0 mmol·L-1 glu-cose and 100 μmol·L-1 AU),the RasAL1 group(cultured with 30.0 mmol·L-1 glucose and 2 μg·L-1 Ras activator RasAL1),and the AU-H+RasAL1 group(cultured with 30.0 mmol·L-1 glucose,100 μmol·L-1 AU,and 2 μg·L-1 RasAL1).All groups were cultured for 48 h.Cell proliferation was assessed by CCK-8 assay;apoptosis rate was evaluated by CalceinAM/PI double staining and flow cytometry;the expression levels of reactive oxygen species(ROS),glutathione per-oxidase(GSH-PX),and malondialdehyde(MDA)were measured using corresponding assay kits;mRNA expression levels of interleukin(IL)-6,IL-1β,and tumor necrosis factor(TNF)-α were detected by qRT-PCR;and the expression levels of Ras/Raf/MEK/ERK pathway-related proteins were examined by Western blot.Results Compared with the blank group,the in-duction group exhibited decreased cell proliferation and GSH-PX level,increased apoptosis rate,elevated ROS and MDA lev-els,upregulated mRNA expression of IL-6,IL-1β,and TNF-α,and enhanced phosphorylation of MEK,Ras,Raf,and ERK.Compared with the induction group,the RasAL1 group showed decreased cell proliferation and GSH-PX level,increased ap-optosis rate,elevated ROS and MDA levels,upregulated mRNA expression of IL-6,IL-1β,and TNF-α,and enhanced phospho-rylation of MEK,Ras,Raf,and ERK,whereas both the AU-L and AU-H groups exhibited increased cell proliferation and GSH-PX level,decreased apoptosis rate,reduced ROS and MDA levels,downregulated mRNA expression of IL-6,IL-1 β,and TNF-α,and diminished phosphorylation of MEK,Ras,Raf,and ERK.Compared with the AU-H group,the AU-H+RasAL1 group showed decreased cell proliferation and GSH-PX level,increased apoptosis rate,elevated ROS and MDA levels,upregulated mRNA expression of IL-6,IL-1β,and TNF-α,and enhanced phosphorylation of MEK,Ras,Raf,and ERK.Compared with the RasAL1 group,the AU-H+RasAL1 group exhibited increased cell proliferation and GSH-PX level,decreased apoptosis rate,reduced ROS and MDA levels,downregulated mRNA expression of IL-6,IL-1β,and TNF-α,and diminished phosphorylation of MEK,Ras,Raf,and ERK.All differences were statistically significant(all P＜0.05).Conclusion AU attenuates high glucose-induced inflammatory injury in RGCs by inhibiting the Ras/Raf/MEK/ERK pathway.关键词
桃叶珊瑚苷/高糖/视网膜神经节细胞/Ras/Raf/MEK/ERK通路Key words
aucubin/high glucose/retinal ganglion cells/Ras/Raf/MEK/ERK pathway分类
医药卫生引用本文复制引用
康健,郑雪,曹朗..桃叶珊瑚苷调节Ras/Raf/MEK/ERK通路对高糖诱导的视网膜神经节细胞炎性损伤的影响[J].眼科新进展,2026,46(9):694-699,6.基金项目
保定市科技计划项目(编号:2141ZF218) （编号:2141ZF218）
