疑难病杂志2026，Vol.25Issue(9)：1038-1042,5.DOI:10.3969/j.issn.1671-6450.2026.09.003
烧伤后并发脓毒症患者血清miR-181c、miR-663b表达水平变化及临床价值
Changes and clinical value of serum miR-181c and miR-663b levels in patients with sepsis after burns
摘要
Abstract
Objective To investigate the changes and clinical value of serum microRNA-181c(miR-181c)and miR-663b levels in patients with sepsis after burns.Methods From January 2019 to April 2024,80 burn patients who developed sepsis after admission to the Department of Burn and Plastic Surgery of Longhua District Central Hospital in Shenzhen were selected as the observation group,and another 80 burn patients who did not develop sepsis were selected as the control group.Pathogenic bacteria were cultured and detected from wound secretions of burn patients.Serum levels of miR-181c and miR-663b were measured using quantitative real-time PCR.Logistic regression was used to analyze the influencing factors for sepsis in burn patients,and ROC curves were drawn to evaluate the diagnostic value of serum miR-181c and miR-663b for sepsis in burn patients.Results Among 80 specimens from patients with sepsis following burns,a total of 94 pathogenic strains were isolated,including 40 strains of Gram-positive bacteria(mainly Staphylococcus aureus and Staphylococcus epider-midis)(42.55%),51 strains of Gram-negative bacteria and 3 strains of fungi(3.19%).Serum levels of miR-181c and miR-663b in the observation group were significantly higher than those in the control group(t=9.476,7.132;both P<0.001).High serum miR-181c and high serum miR-663b levels were independent risk factors for sepsis in patients after burns[OR(95%CI)=2.867(1.377-5.967),3.152(1.422-6.985)].The AUC for predicting sepsis in burn patients was 0.858 for serum miR-181c,0.811 for miR-663b,and 0.938 for the combination of both,with the combined prediction being significantly supe-rior to either alone(Z=2.531,2.486;P=0.018,0.019).Conclusion Serum levels of miR-181c and miR-663b are elevated in patients with sepsis after burns.Combined detection of these two biomarkers has good diagnostic value for sepsis after burns.关键词
烧伤/脓毒症/微小RNA-181c/微小RNA-663b/临床价值Key words
Burns/Sepsis/MicroRNA-181c/MicroRNA-663b/Clinical value分类
医药卫生引用本文复制引用
李维珊,齐德丽,刘坡,刘月明,徐石,林子哲..烧伤后并发脓毒症患者血清miR-181c、miR-663b表达水平变化及临床价值[J].疑难病杂志,2026,25(9):1038-1042,5.基金项目
2025年度广东省中医药局科研项目立项项目(20251329) 2025 Guangdong Provincial Administration of Traditional Chinese Medicine Research Project Approval(20251329) （20251329）
