云南化工2026，Vol.53Issue(9)：32-36,5.DOI:10.3969/j.issn.1004-275X.2026.09.07
穿龙薯蓣多糖和总皂苷的协同提取工艺研究
Study on the Synergistic Extraction Process of Polysaccharides and Total Saponins from Dioscorea nipponica
摘要
Abstract
[Objective]To systematically evaluate the extraction efficiencies of different methods and solvents for polysaccharides and total sapo-nins from Dioscorea nipponica Makino,and to explore the extraction profiles and synergistic optimization strategies for these bioactive compo-nents.[Methods]Traditional heat reflux extraction(HRE),ultrasonic-assisted extraction(UAE),and microwave-assisted extraction(MAE)were applied using water,50%ethanol,and anhydrous ethanol as solvents.The contents of polysaccharides and total saponins were determined.Additionally,reverse supplementary extraction and thin-layer chromatography(TLC)were employed to verify component distribution and extrac-tion characteristics.[Results]Polysaccharide extraction yield was highest in aqueous solvent,with the HRE-water combination achieving the maximum yield of(36.80±0.42)%.Total saponins were best extracted by UAE using50%ethanol,reaching(10.74±0.21)%,significantly outperforming other solvent systems.Reverse supplementary extraction demonstrated that the cumulative saponin yield from stepwise extraction was notably lower than that from one-step co-extraction,indicating the superiority of synergistic extraction strategy.TLC fingerprinting revealed selective extraction patterns related to solvent polarity,consistent with quantitative analysis.[Conclusion]Polysaccharides are optimally extracted using heat reflux with water,whereas total saponins are preferentially recovered via ultrasonic-assisted extraction with 50%ethanol.One-step synergistic extraction is more efficient than sequential extraction.This study provides experimental evidence and process guidance for the efficient multi-component extraction and quality control of Dioscorea nipponica.关键词
穿龙薯蓣/甾体皂苷/多糖/超声波辅助提取/微波辅助提取/溶剂极性/协同提取Key words
Dioscorea Nipponica/Steroidal Saponins/Polysaccharides/Ultrasonic-assisted Extraction/Microwave-assisted Extraction/Solvent Po-larity/Synergistic Extraction分类
医药卫生引用本文复制引用
伏玉洋,张海容..穿龙薯蓣多糖和总皂苷的协同提取工艺研究[J].云南化工,2026,53(9):32-36,5.基金项目
广州华商学院2023 年校级新文科、新工科、新医科研究与改革实践项目(HS2023SX23). （HS2023SX23）
