医学信息2026，Vol.39Issue(17)：40-46,7.DOI:10.3969/j.issn.1006-1959.2026.17.006
基于TLR-4/MyD88/NF-κB通路探讨粪菌移植对多囊卵巢综合征大鼠的治疗作用
Exploring the Therapeutic Effect of Fecal Microbiota Transplantation on Polycystic Ovary Syndrome Rats Based on the TLR-4/MyD88/NF-κB Pathway
摘要
Abstract
Objective To explore the potential mechanism of fecal microbiota transplantation(FMT)in the treatment of polycystic ovary syndrome(PCOS)model rats through TLR-4/MyD88/NF-κB signaling pathway.Methods Thirty female SD rats aged 6-8 weeks were randomly divided into control group,model group and fecal microbiota transplantation group.The PCOS model was established by intragastric administration of trazol for 21 days in the model group.After successful modeling,FMT intervention was performed.The body weight was weighed every 7 days,and the estrous cycle was observed by vaginal smear.The rats were fasted for 12 hours before being sacrificed,and the levels of fasting plasma glucose(FPG),fasting insulin(FINS)and Homeostatic Model Assessment for Insulin Resistance(HOMA-IR)were detected.The levels of testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)and lipopolysaccharide(LPS)in serum were detected by ELISA.HE staining was used to observe the pathological changes of ovarian tissue,and Western Blot was used to detect the expression levels of TLR-4,MyD88 and NF-κB protein in ovarian tissue of rats in each group.Results Compared with the control group,the body weight,ovarian weight and ovarian weight/body weight of the rats in the model group were significantly increased,the estrous cycle was disordered,the ovarian tissue showed multiple follicular changes,the levels of serum T,LH,LH/FSH and HOMA-IR were increased,the level of FSH was decreased,the level of LPS was significantly increased,the levels of pro-inflammatory factors IL-1 β,IL-6 and TNF-α were increased,the level of anti-inflammatory factor IL-10 was decreased,and the expression levels of TLR-4,MyD88 and NF-κB protein were increased(P<0.05).Compared with the model group,the body weight,ovarian weight and ovarian weight/body weight of the rats in the fecal microbiota transplantation group were significantly decreased,the estrous cycle of the rats was significantly restored,the dominant follicles and corpus luteum were observed in the ovarian tissue,the levels of serum T,LH,LH/FSH,HOMA-IR and LPS were decreased,the levels of pro-inflammatory factors IL-1β,IL-6 and TNF-α were decreased,the level of anti-inflammatory factor IL-10 was increased,and the expression levels of TLR-4,MyD88 and NF-κB proteins were decreased(P<0.05).Conclusion Fecal microbiota transplantation can reduce the body weight of PCOS rats,reduce androgen levels,improve HOMA-IR,inhibit TLR-4/MyD88/NF-κB signaling pathway,reduce the expression of LPS and inflammatory factors,improve chronic inflammation and restore ovarian function.关键词
多囊卵巢综合征/粪菌移植/TLR-4/MyD88/NF-κB信号通路/炎症因子Key words
Polycystic ovary syndrome/Fecal microbiota transplantation/TLR-4/MyD88/NF-κB signaling pathway/Inflammatory factor分类
医药卫生引用本文复制引用
迪丽拜尔·安外尔,张曼丽,黄雅楠,张盼,腊晓琳..基于TLR-4/MyD88/NF-κB通路探讨粪菌移植对多囊卵巢综合征大鼠的治疗作用[J].医学信息,2026,39(17):40-46,7.基金项目
1.新疆维吾尔自治区自然科学基金青年科学基金项目(编号:2024D01C277) （编号:2024D01C277）
2.中央引导地方科技发展资金项目(编号:ZYYD2024ZY07) （编号:ZYYD2024ZY07）
3.省部共建中亚高发病成因与防治国家重点实验室联合基金课题资助项目(编号:SKL-HIDCA-2023-KE6) （编号:SKL-HIDCA-2023-KE6）
