医药导报2026，Vol.45Issue(9)：1491-1498,8.DOI:10.3870/j.issn.1004-0781.2026.09.002
大戟脂总三萜对MH7A细胞增殖凋亡迁移和侵袭的影响
Effects of Euphorbium Total Triterpenoids on Proliferation,Apoptosis,Migration,and Invasion of MH7A Cells
摘要
Abstract
Objective To investigate the effects of Euphorbium total triterpenoids(TTE)on the proliferation,apoptosis,migration,and invasion of MH7A cells(rheumatoid arthritis fibroblast-like synoviocytes,RA-FLS).Methods The experimental concentrations of TTE were determined as 6.25,12.5,and 25 μg·mL-1 based on CCK-8 results.Furthermore,cell migration and invasion were evaluated using a scratch wound-healing assay and a Transwell invasion assay,respectively,while apoptosis was detected by flow cytometry.The levels of tumor necrosis factor-alpha(TNF-α),interleukin-1beta(IL-1β),and interleukin-6(IL-6)in the cell culture supernatant were measured using enzyme-linked immunosorbent assay(ELISA).Western blotting assay was used to detect the protein expression of PI3K,p-Akt,p-mTOR,MMP-3,MMP-9,and Cleaved Caspase-3 in MH7A cells.Results Compared with the blank control group,TTE significantly inhibited the proliferation,migration,and invasion of MH7A cells and promoted their apoptosis(P<0.05).TTE(12.5 μg·mL-1)could also effectively reduce the level of TNF-α,IL-1β,and IL-6 in the cell supernatant(P<0.05),inhibit the expression of migration-and invasion-related proteins MMP-3 and MMP-9(P<0.05),and promote the expression of the apoptosis-related protein Cleaved Caspase-3(P<0.05).Moreover,TTE(25 μg·mL-1)could down-regulate the expression of PI3K,p-Akt,and p-mTOR proteins in MH7A cells(P<0.05).Conclusions Preventive mechanisms of TTE in RA involve inhibiting the proliferation,migration,and invasion of MH7A cells,as well as promoting their apoptosis.These findings provide a basis for further development of TTE.关键词
大戟脂总三萜/类风湿关节炎/MH7A细胞/增殖/凋亡/迁移/侵袭Key words
Euphorbium total triterpenoids/Rheumatoid arthritis/MH7A cells/Proliferation/Apoptosis/Migration/Invasion分类
医药卫生引用本文复制引用
刘霞,艾迪达尔·托力肯,李澳文,姚雨含,赵军..大戟脂总三萜对MH7A细胞增殖凋亡迁移和侵袭的影响[J].医药导报,2026,45(9):1491-1498,8.基金项目
国家自然科学基金资助项目(82360764). （82360764）
